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Morphogenesis of the Golgi apparatus and its expression of normal function

高尔基体的形态发生及其正常功能的表达

基本信息

批准号：
06670036
负责人：
YAMASHINA Shohei
金额：
$ 1.34万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The Golgi apparatus of a rat parotid acinar cells is consisted of 4-5 layrs of tightly stacked cisternae, trans Golgi network, an amorphous membranous system that is located in the close portion to the trans-most cistern, and a large number of associated vesicles about 70nm in diameter. Although its cisternal stack shows very complicating feature with folding and branching, this is continuous and one single organelle situated in the supra-nuclear region of the cell. The characteristic cisternal structure can be disorganized reversibly by the use of Brefeldin A,okadaic acid, monensin and anti-microtubular agents. The present study was undertaken to analyze the mechanism of biogenesis and maintenance of the characteristic structure, and relationship of the structure with functional expression of the normal sorting transport of the synthesized proteins in the special stages of destruction and reconstruction after the administration of these drugs. As a result, followings were concluded ;1. Microtubules works to maintain the configuration and positioning of the Golgi apparatus, but it appears not to play a significant role in the morphogenesis of the stacked structure.2. The lamellated structure of the Golgi apparatus is a structural background to mediate sorting transport of the synthesized proteins in the cells.3. The characteristic structure seems to be fundamentally important for the Golgi apparatus to express normal function.These results indicated that the determining factors of the characteristic structure should be analyzes. In this respect, no such factors has been detected as yet, however, molecules that play a role in adhere the each cisternal membrane should be purified, and their molecular mechanism must analyzed by the future study.

大鼠腮腺腺泡细胞的高尔基体由4-5层紧密堆积的池、反式高尔基网络（位于最反式池的近部的无定形膜系统）和大量直径约70nm的相关囊泡组成。虽然它的池堆显示出非常复杂的折叠和分支特征，但这是连续的，一个单一的细胞器位于细胞的核上区。Brefeldin A、冈田酸、莫能菌素和抗微管药物可可逆地破坏其特有的蓄水池结构。本研究旨在分析该特征结构的生物发生和维持机制，以及该结构与合成蛋白在给药后的特殊破坏和重建阶段正常分选转运功能表达的关系。结果得出以下结论：微管的作用是维持高尔基体的结构和定位，但它在堆叠结构的形态形成中似乎没有发挥重要作用。高尔基体的层状结构是介导合成蛋白在细胞内分选转运的结构背景。特征结构似乎对高尔基体表达正常功能至关重要。这些结果表明，需要对特征结构的决定因素进行分析。在这方面，目前还没有发现这样的因素，但是，在每个池膜粘附中起作用的分子需要纯化，其分子机制必须在未来的研究中分析。