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Studies on the Identification and Differentiation of Tissue Stem Cells in Exocrine Organs by 3-D Immunohistochemistry

3-D免疫组织化学鉴定和分化外分泌器官组织干细胞的研究

基本信息

批准号：
18590193
负责人：
YAMASHINA Shohei
金额：
$ 2.34万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

1. Identification of tissue stem cells in the salivary gland In the rat parotid gland after repeated injection by isoproterenol, differentiation potential was studied by means of multiple immunohistochemistry. As a result, followings were concluded. (1) Terminal cells in the intercalated duct differentiate toward acinar cells at 3rd days after IPR treatment. (2) Differentiation was not demonstrated in cells in other portion of intercalated duct by the experimental system.2. Differntiation potential of intercalated duct cells after the partial pancreatectomy During the course of regeneration after the partial pancreatectomy, 3-D reconstruction was performed from serial section after the treatment of multiple immunohistochemistry of cytokeratin 20 and insulin. As a result, it was found that cells at the terminal portion of intercalated duct differentiated toward acinar cells and cells at the branching portion of intercalated duct differentiated into endocrine cells.3. Determination of endocrine stem cells in the developing rat pancreas Development of pancreatic islets was investigated by means of 3-D reconstruction treated by multiple immunohistochemistry. Glucagon positive cells were found to appear in the various portion of tubulo-acinar structure during 15 to 17 days in embryonic days, and plural endocrine cells gathered to form a single islet, indicating that the endocrine cells in a islet were not a clone but multiple origin.

1.唾液腺组织干细胞的鉴定在重复注射异丙肾上腺素后的大鼠腮腺中，通过多重免疫组化方法研究分化潜能。结果，得出以下结论。 (1) IPR处理后第3天，闰管终末细胞向腺泡细胞分化。 (2)实验系统未显示闰管其他部分细胞的分化。2．部分胰腺切除术后闰管细胞的分化潜能在部分胰腺切除术后的再生过程中，在细胞角蛋白20和胰岛素的多重免疫组化处理后，从连续切片进行3-D重建。结果发现，闰管末端细胞向腺泡细胞分化，闰管分支部细胞向内分泌细胞分化。 3．测定发育中的大鼠胰腺中的内分泌干细胞通过多重免疫组织化学处理的 3-D 重建来研究胰岛的发育。在胚胎期15～17天，发现胰高血糖素阳性细胞出现在肾小管腺泡结构的各个部分，并且多个内分泌细胞聚集形成单个胰岛，表明胰岛中的内分泌细胞不是克隆而是多个来源。