Immunohistochemical study on the structure and function of the ductal system of digestive gland

消化腺导管系统结构与功能的免疫组化研究

• 批准号: 08670031
• 负责人: YAMASHINA Shohei
• 金额: $ 1.22万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670031/
• 关键词: Digestive gland; Duct system; Regeneration; Development; Cell proliferation; Apoptos is; Immunohistochemistry; EGF; 外分泌腺; 導管; 細胞分裂; アポトシース; PCNA; TUNEL

(1) Identification of Kalliklein containing cell in the salivary duct systemBy immunoelectronmicroscopy, kalliklein was localized in small vesicles of striated duct cells, of which secretion was induced by alpha-adrenergic and cholinergic stimuli.(2) Detection of proliferating activity in the exocrine duct cellsThe BrdU method indicated that DNA synthetic activity was the highest in acinar and cetroacinar cells of the rat pancreas, and these cells were suggested to be potential proliferating cells.(3) Cellular proliferation and apoptosis in the developing rat parotid glandProliferation and apoptosis was investigated in the developing parotid gland by immunohistochemical method. As a result, branching morphogenesis of parotid gland was suggested to proceed by proliferation of cells solely located in the terminal portion of immature duct system, and during the course no apoptosis was found to take place.(4) Cell kinetics in regeneration after partial pancrectomyPancreas of rat was partia … More lly removed and proliferating activity during the course of regeneration was studied. From 3 days after the operation, proliferation started in the acinar portion which continued until 2 weeks. Compensation of cells by regeneration was concluded to take place by the proliferation of terminal cells. This result was in good agreement with the one described in (2).(5) Effect of EGF in the branching morphogenesisBranching morphogenesis of embryonic mouse submandibular gland was accerelated by EGF,but EGF inhibitor was found to suppress the course. And EGF receptor was localized in the basal portion of the ductal cells at the particular stage of morphogenesis. From these observation, EGF was concluded to play an important role in the branching morphogenesis.(6) Ultrastructure of salivary basement membraneBasement membrane has been indicated to be the site of epithelial-mesenchymal interaction of morphogenesis, since its ultra-fine structure was investigated by high resolution electron microscopy. As-a result, salivary basement membrane was uniform and fine reticular struture of about 40-100nm thickness with vertical fibrils of 100nm diameter. Lamina rara was very likely to be an artificial structure due to fixation. Less

(1)唾液管系统含钾钾素细胞的鉴定通过免疫电镜观察，钾钾素定位于横纹管细胞的小泡中，其分泌受α -肾上腺素能和胆碱能刺激。(2)外分泌管细胞的增殖活性检测BrdU法显示，大鼠胰腺腺泡细胞和鲸泡细胞的DNA合成活性最高，提示这些细胞是潜在的增殖细胞。(3)大鼠腮腺发育过程中的细胞增殖与凋亡采用免疫组织化学方法研究了大鼠腮腺发育过程中的细胞增殖与凋亡。结果表明，腮腺的分支形态发生是由未成熟导管系统末端的细胞增殖进行的，在此过程中未发现细胞凋亡的发生。(4)研究了大鼠胰腺部分切除后再生过程中的细胞动力学。从术后3天开始，腺泡部分开始增殖，并持续到2周。再生对细胞的补偿是通过终端细胞的增殖来实现的。(5) EGF在分支形态发生中的作用EGF可加速小鼠胚胎颌下腺的分支形态发生，但发现EGF抑制剂可抑制这一过程。EGF受体在形态发生的特定阶段定位于导管细胞的基底部。由此得出EGF在分支形态发生中起重要作用的结论。(6)唾液腺基底膜的超微结构通过高分辨电镜观察其超微结构，表明基底膜是上皮-间质相互作用的形态发生部位。唾液基底膜呈均匀的细网状结构，厚度约为40 ~ 100nm，垂直的原纤维直径为100nm。由于固定，椎板很可能是一个人工结构。少

项目成果

Edward G.Gresik,Masanori Kashimata,Yuichi Kadoya,Robin Mathews,Naomi Minami,Shohei Yamashina: "Expression of epdermal growth factor receptor in fetal mouse submandibular glard detected by a biotinyltyramide-based catalyzed signal amplification method." J.

Edward G.Gresik、Masanori Kashimata、Yuichi Kadoya、Robin Mathews、Naomi Minami、Shohei Yamashina：“通过基于生物素酪酰胺的催化信号放大方法检测胎儿小鼠颌下腺中表皮生长因子受体的表达。”

Hideaki Tamaki, Shohei Yamashina: "Three-dimensional dynamics of the Golgi apparatus in mitotic parotid acirar cells:Computer-aided recorstruction from cytochemically-marked ultrathin serial sections." A.Histochem.Cytochem.(発表予定). (1998)

Hideaki Tamaki、Shohei Yamashina：“有丝分裂腮腺腺泡细胞中高尔基体的三维动力学：细胞化学标记的超薄连续切片的计算机辅助重建。”（即将发表）。

Hideaki Tamaki,Shohei Yamashina: "Three-dimesional dynamics of the Golgi apparatus in mitotic parotid acinar cells:Computer-aided reconstruction from cytochemically-marked ultrathin serial sections." A.Histochem.Cytochem.(発表予定). (1998)

Hideaki Tamaki、Shohei Yamashina：“有丝分裂腮腺腺泡细胞中高尔基体的三维动力学：细胞化学标记的超薄连续切片的计算机辅助重建。”（即将发表）。