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On the Determinative Factors of the Feature and Position of Golgi Apparatus

论高尔基体特征和位置的决定因素

基本信息

批准号：
01570017
负责人：
YAMASHINA Shohei
金额：
$ 1.41万
依托单位：
Kitasato University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

In the parotid acinar cells of a rat, Golgi apparatus developed immediately after the birth as a cluster of small vesicles containing cis elements as revealed by osmium impregnation, followed by an appearance of straight cisternae in the vesicular cluster. The stacks increased in number and trans nature as demonstrated by TPPase histochemistry appeared at first in the stacked cisternae. The first appearance of secretory granules was in this stage of development, indicating that the morphological development preceded to functional one. The 3-dimensional analysis by computer graphics from serial section electron micrographs indicated the Golgi apparatus to always be a single cluster in a given acinar cell.Immunohistochemical study by anti-tubulin antibody during the development of acinar cells demonstrated that the microtubules started to appear at the 3rd day after birth, when the extensive development of the Golgi stack are being taken place. The result pointed out that the positioning and the maintenance of characteristic feature of the Golgi apparatus have an intimate relationship with the microtubules.During the mitosis that induced by the isoproterenol, the Golgi apparatus become fragment containing cis elements but not trans one. The process of reassembly taken plaace in the daughter cells is quite similar to that of developmental course. The administration of brefeldin A, an agent to block the transport of secretory proteins from rough ER to Golgi, produced a marked regression of Golgi apparatus into aggregation of small vesicles containing cis elements. The continuity of these clusters was again obvious by computer graphics.These results pointed out that the structurally principal region of the Golgi is in ciselements, to which medial and trans elements are added according to functional demand. The principal regions appears to be bound into a single structure by the function of microtubules.

在大鼠腮腺腺泡细胞中，高尔基体在出生后立即发育为一簇含有顺式元件的小泡，如锇浸渍所揭示的，随后在泡簇中出现直池。TPPase组织化学显示，堆叠的数量和反式性首先出现在堆叠的池中。分泌颗粒在此发育阶段首次出现，表明形态发育先于功能发育。电镜连续切片的计算机三维分析表明，腺泡细胞中的高尔基体始终是一簇，腺泡细胞发育过程中微管蛋白抗体的免疫组化研究表明，生后3天开始出现微管，此时高尔基体正在广泛发育。结果表明，高尔基体特征的定位和维持与微管有密切的关系，在异丙肾上腺素诱导的有丝分裂过程中，高尔基体变成含有顺式元件而不含反式元件的片段。子细胞中发生的重组过程与发育过程非常相似。布雷菲德菌素A，一种药物，以阻止运输的分泌蛋白质从粗糙的ER到高尔基体，产生了显着的回归高尔基体成聚集的小泡含有顺式元件。这些结果表明，高尔基体结构上的主要区域是顺式元件，根据功能的需要，在顺式元件中加入了中间和反式元件。主要区域似乎通过微管的功能结合成单一结构。