SERUM LIPID PEROXIDES,SERUM ALKENALS AND GLUTATHIONE PEROXIDASE IN SERUM AND ERYTHROCYTE,AND THE EFFECTS OF LIFE STYLE

我们研究了这种生活方式的影响，如血清脂质过氧化物水平和血清中谷氨酸过氧化物酶（GPX）的活性，以及血清和红细胞中的食物摄入、饮酒和吸烟习惯。一种用于确定aliphaldchydes（alkenals）的荧光测量方法，是从生物样品中开发的脂质过氧化物中分解的产品，使用1，3-环己二酮试剂和高压力液体色谱法。使用这个方法，我们测量了血清脂质过氧化物（TBARS）和在健康工作者中的烯烃。我们还利用Paglia等人的修改方法测量了血清和红细胞中GPX中GPX的抗体，其中包括325个工人，随机从工厂约7，000名雇员中选择了325个工人。1.血清TBARS的平均值和标准配置4.8（]SY.+- [）男性2.1 nmol/ml和5.3（]SY.+- [1]妇女中2.9 nmol/ml。对于alkenals来说，一般和肛门的意义和标准偏差是相当的1.17（]SY.+- [1]男性0.66微克/毫升，0.99微克/毫升 ... More Y.+- [）0.47微克/毫升， 0.10（]SY.+- [）0.01 mu g/ml in men and 0.08（]SY.+- [）0.04微克/毫升的血清TBARS和烯烃与BMI、血、血清脂质和氧的逃逸活动的水平.3.血清TBARS之间的某些关系和营养元素的营养量之间的平衡和营养元素之间的平衡和营养元素的营养量之间的平衡和营养元素的营养量之间的平衡和营养元素的营养量之间的平衡和营养元素的营养量.4.轻和重的饮酒者展示了比血清TBARS更高的水平。但在吸烟者和非吸烟者之间没有发现差异。5. GPX在血清和血清细胞中男性对象中的血清和红细胞的活动的标准特征是相应的389（]SY.+- [）42 DELTA nmolNADPH/min·ml和59.3（]SY.+- [）6.2 DELTA nmolNADPH/min·mg·Hb. 6.香烟吸烟者展示了血清中GPX较低的活动。对变态细胞进行监管，GPX的活动并不受吸烟习惯的影响。由于饮酒习惯的影响，GPX在饮酒者和非饮酒者之间的活动没有区别。但重的饮酒者展示了GPX在红细胞中的活动比轻饮料者更高。7. GPX在血清中的活动与HDL-胆固醇有关。Less（低）

We investigated the effect of life style such as food intake, drinking and smoking habits on the levels of serum lipid peroxides and on the activities of glutathione peroxidase (GPX) in serum and erythrocyte. A fluorometric method for determination of aliphatic aldchydes (alkenals), which are decomposed products from lipid peroxides in biological samples was developed, using 1,3-cyclohexanedione reagent and high pressure liquid chromatography. Using this method, we measured serum lipid peroxides (TBARS) and alkenals in healthy workers. We also measured the antivities of GPX in serum and erythrocyte using the modified method of Paglia et al. The subjects comprised 325 workers selected at random from about 7000 employees at a factory.1.The means and standard deviations of serum TBARS were 4.8(]SY.+-[)2.1 nmol/ml in men and 5.3(]SY.+-[)2.9 nmol/ml in women. For alkenals, the means and standard deviations of propanal and butanal were respectively 1.17(]SY.+-[)0.66 mu g/ml in men and 0.99(] … More SY.+-[)0.47 mu g/ml in women, 0.10(]SY.+-[)0.01 mu g/ml in men and 0.08(]SY.+-[)0.04 mu g/ml in women.2.The levels of serum TBARS and alkenals had correlations with BMI,blood, serum lipids and oxygen escape activity from hepatocytes.3.Ther were some relations between serum TBARS and the amount of dietary intakes of nutritional elements, and between alkenals and the amount of dietary intakes of nutritional elements.4.Light and heavy drinkers showed a higher level of serum TBARS than non-drinkers. But no differences was found between smokers and non-smokers.5.The means and standard deviations of the activities of GPX in serum and erythrocyte of male subjects were respectively 389(]SY.+-[)42 DELTA nmolNADPH/min・ml and 59.3(]SY.+-[)6.2 DELTA nmolNADPH/min・mg・Hb.6.Cigarette smokers showed a lower activity of GPX in serum than non-smokers. Regarding erythrocyte, the activity of GPX was not affected by smoking habits. As for effect of drinking habits, there was no difference in the activity of GPX in serum between drinkers and non-drinkers. But heavy drinkers showed a higher activity of GPX in erythrocyte than light drinkers.7.THe activity of GPX in serum was correlated with HDL-cholesterol in serum. Less