SERUM LIPID PEROXIDES,SERUM ALKENALS AND GLUTATHIONE PEROXIDASE IN SERUM AND ERYTHROCYTE,AND THE EFFECTS OF LIFE STYLE

血清和红细胞中的血清过氧化物、血清烯醛和谷胱甘肽过氧化酶以及生活方式的影响

• 批准号: 06670438
• 负责人: NAKAGAWA Hideaki
• 金额: $ 1.34万
• 依托单位: KANAZAWA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670438/
• 关键词: Lipid peroxides; alkenals; glutathione peroxidase activity; blood pressure; serum lipid; atherosclerosise; life style; チオバルビタール酸; 栄養素摂取量; 精神ストレス; 高血圧; 尿中電解質

We investigated the effect of life style such as food intake, drinking and smoking habits on the levels of serum lipid peroxides and on the activities of glutathione peroxidase (GPX) in serum and erythrocyte. A fluorometric method for determination of aliphatic aldchydes (alkenals), which are decomposed products from lipid peroxides in biological samples was developed, using 1,3-cyclohexanedione reagent and high pressure liquid chromatography. Using this method, we measured serum lipid peroxides (TBARS) and alkenals in healthy workers. We also measured the antivities of GPX in serum and erythrocyte using the modified method of Paglia et al. The subjects comprised 325 workers selected at random from about 7000 employees at a factory.1.The means and standard deviations of serum TBARS were 4.8(]SY.+-[)2.1 nmol/ml in men and 5.3(]SY.+-[)2.9 nmol/ml in women. For alkenals, the means and standard deviations of propanal and butanal were respectively 1.17(]SY.+-[)0.66 mu g/ml in men and 0.99(] … More SY.+-[)0.47 mu g/ml in women, 0.10(]SY.+-[)0.01 mu g/ml in men and 0.08(]SY.+-[)0.04 mu g/ml in women.2.The levels of serum TBARS and alkenals had correlations with BMI,blood, serum lipids and oxygen escape activity from hepatocytes.3.Ther were some relations between serum TBARS and the amount of dietary intakes of nutritional elements, and between alkenals and the amount of dietary intakes of nutritional elements.4.Light and heavy drinkers showed a higher level of serum TBARS than non-drinkers. But no differences was found between smokers and non-smokers.5.The means and standard deviations of the activities of GPX in serum and erythrocyte of male subjects were respectively 389(]SY.+-[)42 DELTA nmolNADPH/min・ml and 59.3(]SY.+-[)6.2 DELTA nmolNADPH/min・mg・Hb.6.Cigarette smokers showed a lower activity of GPX in serum than non-smokers. Regarding erythrocyte, the activity of GPX was not affected by smoking habits. As for effect of drinking habits, there was no difference in the activity of GPX in serum between drinkers and non-drinkers. But heavy drinkers showed a higher activity of GPX in erythrocyte than light drinkers.7.THe activity of GPX in serum was correlated with HDL-cholesterol in serum. Less

本文探讨了饮食、饮酒、吸烟等生活方式对血清脂质过氧化物水平及血清和红细胞谷胱甘肽过氧化物酶（GPX）活性的影响。建立了用1，3-环己二酮试剂和高压液相色谱法测定生物样品中脂质过氧化物分解产物脂肪醛（烯醛）的荧光分析方法。用此方法测定了健康工人血清过氧化脂质（TBARS）和醛类物质。用改良的Paglia等方法测定了某厂7000名职工中随机抽取的325名工人血清和红细胞GPX抗体。[)2.1男性为5.3 ± 0.5nmol/ml，[)2.9 nmol/ml。对于烯醛，丙醛和丁醛的平均值和标准偏差分别为1.17（]SY. ±- []男性为0.66 μ g/ml， ...更多信息 SY.+-女性0.47 μ g/ml，女性0.10 μ g/ml，[）男性0.01微克/毫升，0.08（]SY.+- 2.血清TBARS和烯醇化酶水平与BMI、血脂、肝细胞氧逃逸活性有关; 3.血清TBARS和烯醇化酶与膳食营养素摄入量有关; 4.轻度和重度饮酒者血清TBARS水平均高于不饮酒者。5.男性受试者血清和红细胞中GPX活性的均值和标准差分别为389（± SD）. ± SD. [)42Δ nmolNADPH/min·ml和59.3（± SD）[)6.2Δ nmolNADPH/min·mg·Hb。6.吸烟者血清GPX活性低于不吸烟者。红细胞GPX活性不受吸烟习惯的影响。饮酒习惯对血清GPX活性的影响，饮酒者与不饮酒者之间无差异。但大量饮酒者红细胞GPX活性高于轻度饮酒者。7.血清GPX活性与血清HDL-胆固醇水平呈正相关。少