SIGNIFICATION OF VOLATILE COMPOUNDS DETECTED IN BODY TISSUES

身体组织中检测到的挥发性化合物的意义

• 批准号: 06670457
• 负责人: KIMURA Kojiro
• 金额: $ 1.34万
• 依托单位: SHIMANE MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670457/
• 关键词: Forensic toxicology; Kerosene; Gas chromatography; mass spectrometry; Trimethylbenzene; Body tissue

1) Determination of kerosene componentsA sensitive and rapid method to analyze kerosene components was developed by making use of capillary gas chromatography/mass spectrometry (GC/MS) combined with the solvent extraction method using n-pentane.The aliphatic hydrocarbons with carbon number over 9 and aromatics such as toluene, xylene and trimethylbenzenes were clearly detected in body tissues exposed to kerosene vapor. The lower limit of detection was ca. 10 pg in each of trimetylbenzenes.These result suggest that this method is useful to detect fuel components in body tissues.2) Relationship between the levels of kerosene components in body tissues and the concentration of kerosene vapor inhaledRats were exposed to kerosene vapor (1,000 ppm) in a chamber made by using common glass bottles and tubes for 5 or 15 min. Trimethylbenzenes including cumen, puseudocumene, mesitylene and 1,2,3-trimethylbenzene in tissues were selected as indicators to presume the concentration of kerosene vapor exposed. Blood levels of the four compounds increased in proportion to the inhalation time of kerosene vapor, and almost the same tendency was observed in the other body tissues. The concentrations of trimethylbenzenes in the lung were nearly the same as those in the blood either for 5 or 15 min-exposure to kerosene, so that the lung would be the most substitutive sample when blood could not be collected due to decomposition.

1)煤油组分的测定采用毛细管气相色谱/质谱（GC/MS）结合正戊烷溶剂萃取法，建立了一种灵敏、快速的煤油组分分析方法，在暴露于煤油蒸气的人体组织中，能清晰地检测出碳数大于9的脂肪烃和芳烃，如甲苯、二甲苯和三甲基苯。检测下限约为0.05 μ g/ml。结果表明，该方法可用于检测人体组织中的燃料成分。2）人体组织中煤油成分含量与吸入煤油蒸气浓度的关系（1，000 ppm）在通过使用普通玻璃瓶和管制成的室中持续5或15分钟。三甲苯包括枯烯、假枯烯、均三甲苯和1，2，以组织中的间三甲苯为指标，推测煤油蒸气暴露浓度。这四种化合物的血液水平与煤油蒸气的吸入时间成比例地增加，并且在其他身体组织中观察到几乎相同的趋势。肺中三甲苯的浓度与血液中的浓度几乎相同，无论是在5或15分钟暴露于煤油，因此，肺将是最替代的样品时，血液不能收集由于分解。