ANALYSIS OF β-PHENYLETHYLAMINE AND CHANGES OF ITS LEVEL IN THE BRAIN AFTER ADMINISTRATION OF METHAMPHETAMINE

β-苯乙胺分析及其服用甲基苯丙胺后脑内水平的变化

• 批准号: 10670390
• 负责人: KIMURA Kojiro
• 金额: $ 2.05万
• 依托单位: SHIMANE MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670390/
• 关键词: Forensic toxicology; Neuro toxicology; Gas chromatography; mass spectrometry; Microdialysis; β-Phenylethylamine; Methamphetamine; β-Phenylethylamine; 質量分析計; 免疫組織化学

The structure is similar to dopamine and stimulant drug on β-phenyl-ethylamine (PEA) which is an endogenous chemical compound. The change of the PEA concentration in the b rain may be concerned in the mental retardation expression, while PEA affects it as a dopaminergic neurotoxin, and influence of stimulant drugs on PEA also seems to be very important in order to evaluate nerve cell change behavioral effects, etc..In this study, we measured the basic value of PEA concentration in extracellular fluid obtained from rat striatum by using microdialysis method combined with gas chromatography/mass spectrometry. Simultaneously, the changes of PEA concentration in various parts of the brain after methamphetamine (MA) administration was also examined.1) A sensitive and reliable analytical method was established by means of 3-phenyl-1-propylamine as an internal standard and pentafluorobenzaldehyde as a derivative agent. The basal level of PEA in extracellular fluid of rat striatum was around 260.54 pg/ml.2) Extracellular PEA level in rat striatum gradually increased with a slight decrease at the time of 24th after administration of 20 mg/kg of MA. This finding indicated that the competitive antagonization of monoamine oxydase B by amphetamine which is a potent metabolite of MA or inhibition of reup take of PEA would occur during the examination. The basal levels of PEA in intracellular fluid were slightly higher in the striatum and hypothalamus than the other parts of the brain. The PEA concentrations in the striatum, hippocampus, hypothalamus, mesencephalon and pons, etc. were gradually decreased after administration of MA, so that the micro-degeneration of neural cells would generate by MA administration. On the other hand, no remarkable changes In histo-pathological examinations were observed in the various parts of the brains at the time of 6 or 12hr after 10 mg/kg of MA administration.

其结构类似于内源性化合物β-苯乙胺(PEA)上的多巴胺和兴奋剂。脑内PEA浓度的变化可能与精神发育迟滞的表达有关，而PEA作为一种多巴胺能神经毒素影响其表达，而兴奋剂对PEA的影响似乎也非常重要，以评估神经细胞的改变行为效应等。1)以3-苯基-1-丙胺为内标，五氟苯甲醛为衍生化试剂，建立了灵敏、可靠的分析方法。大鼠纹状体细胞外液中PEA的基础水平约为260.54 pg/ml2)大鼠纹状体细胞外液中PEA水平逐渐升高，在给药后24小时略有下降。这一发现表明，在检测过程中，MA的有效代谢物苯丙胺可能会对单胺氧合酶B产生竞争性抗凝作用或抑制PEA的再摄取。细胞内液中PEA的基础水平在纹状体和下丘脑略高于脑的其他部分。给予MA后，纹状体、海马、下丘脑、中脑、脑桥等部位的PEA浓度逐渐降低，从而导致神经细胞的微变性。而在10 mg/kg给药后6小时或12小时，脑内各部位的组织病理学检查均未见明显改变。