TOXICOLOGICAL ANALYSIS FOR ABUSED VOLATILE COMPOUNDS

滥用挥发性化合物的毒理学分析

• 批准号: 02670262
• 负责人: KIMURA Kojiro
• 金额: $ 1.47万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670262/
• 关键词: forensic toxicology; body tissues; gas chromatography; mass spectrometry; kerosene; light oil; thinner; oral intake; postmortem changes; プソイドクメン; 揮発性物質; ガスクロマトグラフィ-; 気化平衡法; 塩析効果; シンナ-

1) Determination of kerosene and light oilA sensitive and rapid method to analyze fuel components in blood from rats exposed to kerosene or light oil vapor was developed by making use of capillary gas chromatography/mass spectrometry(GC/MS).The aliphatic hydrocarbons with carbon numbers 8 to 10 and aromatics such as toluene,xylene,3- and 4-ethyltoluene and trimethylbenzenes were clearly detected in blood from rats exposed to kerosene or light oil vapor,using the head space method combined with the salting-out technique. The concentration ratio of pseudocumene to toluene in blood exposed to light oil was higher than that in case of exposure to kerosene. The lower limits of detection were 50 pg and 1 ng in toluene and pseudocumene, respectively.Our suggestion is that this method is useful in forensic investigations to detect fuel components in blood and for the purposes of differentiating kerosene and light oil in blood tissues. 2) Postmortem changes of ingested thinnerPostmortem changes … More of thinner components in body tissues were examined in rats, orally given 1 ml of standard thinner solution, which was prepared by mixing toluene, ethyl acetate and isobutanol in the proportion of 8:1:1. Analysis was made by gas chromatography combined with the head space method. Three thinner components and ethanol, a metabolite of ethyl acetate, were detected in the gastric contents of all rats up until 48 hours after death. An increase in the concentration of toluene was found in the blood,lung,kidney,liver,brain and abdominal muscle with the lapse of time. On the other hand,no changes were observed in the thigh muscle throughout the 48-hour period. Isobutanol showed a similar increasing pattern to toluene,with little or no changes in the brain or in the thigh muscle. Ethyl acetate was not detected in any tissues throughout the study but it was found in the gastric contents.The results indicate that every thinner component ingested,gradually diffuses into the surrounding tissues through the stomach wall after death,and that only muscle tissue remote from the abdominal cavity,together with the gastric contents,should be analyzed for a correct diagnosis of thinner ingestion. Less

1)煤油和轻油的测定采用毛细管气相色谱/质谱(GC/MS)技术建立了一种灵敏、快速分析接触煤油或轻油蒸汽的大鼠血液中燃料成分的方法。其中碳原子数为8~10的脂肪烃和芳香族化合物如甲苯、二甲苯、3-和4-乙基甲苯和 采用顶空法结合盐析技术，在接触煤油或轻油蒸气的大鼠血液中清晰地检出了三甲苯。接触轻油的血液中假枯烯与甲苯的浓度比高于接触煤油的情况。甲苯和假异丙苯的检测下限分别为 50 pg 和 1 ng。我们的建议是，该方法可用于法医学研究以检测血液中的燃料成分以及区分血液组织中的煤油和轻油。 2）摄入稀释剂的死后变化 大鼠体内组织中稀释剂成分的死后变化，口服1ml标准稀释剂溶液，该标准稀释剂溶液是由甲苯、乙酸乙酯和异丁醇按8：1：1的比例混合而成。采用气相色谱法结合顶空法进行分析。直至死后 48 小时，所有大鼠的胃内容物中均检测到三种稀释剂成分和乙醇（一种乙酸乙酯的代谢物）。随着时间的推移，血液、肺、肾、肝、脑和腹肌中甲苯的浓度逐渐升高。另一方面，在整个 48 小时内，大腿肌肉没有观察到任何变化。异丁醇表现出与甲苯相似的增加模式，但大脑或大腿肌肉几乎没有变化。在整个研究过程中，没有在任何组织中检测到乙酸乙酯，但在胃内容物中发现了它。结果表明，摄入的每种稀释剂成分在死亡后逐渐通过胃壁扩散到周围组织中，只有远离腹腔的肌肉组织和胃内容物才应该分析，以正确诊断稀释剂摄入。较少的

项目成果

K.Kimura: "Determination of Kerosene and Light Oil Components in Blood" Biological Mass Spectrometry. 20. 493-497 (1991)

K.Kimura：“血液中煤油和轻油成分的测定”生物质谱法。

K.KIMURA: "Postmortem Chamges of Ingested Thinner Components in Tissues" Jpn.J.Legal Med.45(3). 222-226 (1991)

K.KIMURA：“组织中摄入的较薄成分的死后变化”Jpn.J.Legal Med.45(3)。

K.Kimura: "Postmortem Changes of Ingested Thinner Components in Tissues" Jpm.J.Legal.Med.45. 222-226 (1991)

K.Kimura：“组织中摄入的稀释成分的死后变化”Jpm.J.Legal.Med.45。

KIMURA K: "Detection of kersene and light oil components in blood." Biol.mass.spectrom. 20. 493-497 (1991)

KIMURA K：“血液中煤油和轻油成分的检测。”

KIMURA K: "Postmortem changes of ingested thinner components in tissues." Jpn J Legal Med. 45(3). 222-226 (1991)

KIMURA K：“组织中摄入的稀释剂成分的死后变化。”