POSTMORTEM CHANGES OF VOLATILE COMPOUNDS DETECTED IN DECOMPOSED TISSUES

1.采用毛细管气相色谱/质谱联用技术，结合正戊烷溶剂萃取法，建立了一种快速、灵敏地分析腐烂组织中煤油成分的方法。将大鼠暴露在1000ppm的煤油蒸气中15分钟。穿刺后，将大鼠置于室温下0 ~ 24小时。死亡后24 h，组织中仍能明显检测到碳数为9 ~ 20的脂肪烃和三甲基苯，如异丙烯、伪烯、间三甲苯和1,2,3-三甲基苯。死后分解组织中煤油组分含量的变化1)与初始水平相比，组织中三甲基苯的浓度变化幅度在40%至110%之间。三甲基苯在肾、肝、脑等脂肪丰富的器官中的含量从死后6小时开始逐渐升高，呈死后分布。2)被测人群肺血三甲基苯浓度比在0.4 ~ 1.4之间。3)大腿肌肉中三甲基苯的含量在死亡后24小时内基本稳定，因此可以从大腿肌肉中三甲基苯的含量推断出由于分解而无法校正的血液中煤油成分的浓度。

1.Determination of kerosene components in putrefied tissuesA sensitive and rapid method to analyze derosene components in decomposed tissues was developed by making use of capillary gas chromatography/mass spectrometry (GC/MS) combined with the solvent extraction method using n-pentane.Rats were exposed to 1,000 ppm kerosene vapor for 15 min. After pithing, the rats were left at room temperature during from 0 to 24 hrs. The aliphatic hydrocarbons with carbon number from 9 to 20 and trimethylbenzenes such as cumene, pseudocumene, mesitylene and 1,2,3-trimethylbenzene were clearly detected in tissues until 24 hrs after death.2.Postmortem changes of levels of kerosene components in decomposed tissues1) The concentrations of trimethylbenzenes in tissues were changed in a range of 40 to 110% against the initial levels. The level of trimethylbenzenes in fat riched organ such as kidney, liver and brain were gradually increased from 6 hrs after death due to postmortem distribution.2) The concentration ratios of trimethylbenzenes in lung to blood were from 0.4 to 1.4 within the examined.3) The levels of trimethylbenzens in thigh muscle were nearly stable within 24 hrs after death, so that the concentration of kerosene components in blood which could not have been corrected due to decomposition would be inferred from the values of trimethylbenzines in thigh muscle.