POSTMORTEM CHANGES OF VOLATILE COMPOUNDS DETECTED IN DECOMPOSED TISSUES

腐烂组织中检测到的挥发性化合物的死后变化

• 批准号: 08670488
• 负责人: KIMURA Kojiro
• 金额: $ 1.41万
• 依托单位: SHIMANE MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670488/
• 关键词: Forensic toxicology; Postmortem change; Gas chromatography; mass; Kerosene; Trimethylbenzene; 腐敗組織

1.Determination of kerosene components in putrefied tissuesA sensitive and rapid method to analyze derosene components in decomposed tissues was developed by making use of capillary gas chromatography/mass spectrometry (GC/MS) combined with the solvent extraction method using n-pentane.Rats were exposed to 1,000 ppm kerosene vapor for 15 min. After pithing, the rats were left at room temperature during from 0 to 24 hrs. The aliphatic hydrocarbons with carbon number from 9 to 20 and trimethylbenzenes such as cumene, pseudocumene, mesitylene and 1,2,3-trimethylbenzene were clearly detected in tissues until 24 hrs after death.2.Postmortem changes of levels of kerosene components in decomposed tissues1) The concentrations of trimethylbenzenes in tissues were changed in a range of 40 to 110% against the initial levels. The level of trimethylbenzenes in fat riched organ such as kidney, liver and brain were gradually increased from 6 hrs after death due to postmortem distribution.2) The concentration ratios of trimethylbenzenes in lung to blood were from 0.4 to 1.4 within the examined.3) The levels of trimethylbenzens in thigh muscle were nearly stable within 24 hrs after death, so that the concentration of kerosene components in blood which could not have been corrected due to decomposition would be inferred from the values of trimethylbenzines in thigh muscle.

1.采用毛细管气相色谱/质谱（GC/MS）结合正戊烷溶剂萃取法，建立了一种快速、灵敏地分析腐败组织中煤油成分的方法。尸体组织中碳原子数为9 ~ 20的脂肪烃和三甲苯类化合物，如枯烯、偏枯烯、均三甲苯和1，2，3-三甲苯，在死后24小时内仍能检测到。2.尸体分解组织中煤油成分含量的变化1）三甲苯类化合物在尸体组织中的含量变化范围为40 ~ 110%。三甲苯类化合物在肝脏、肾脏和脑等脂肪组织中的含量从死后6 h开始逐渐增加，这是由于死后分布的原因。2）肺中三甲苯类化合物与血液中三甲苯类化合物的浓度比值在0.4 ~ 1.4之间。3）大腿肌肉中三甲苯类化合物的含量在死后24 h内基本稳定。因此，血液中由于分解而无法校正的煤油成分的浓度将从大腿肌肉中的三甲基苯值推断出来。