Forensic Analysis of Fuel Components in Body Materials

车身材料中燃料成分的法医分析

• 批准号: 62570267
• 负责人: KIMURA Kojiro
• 金额: $ 1.22万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570267/
• 关键词: Fuel component; Gas chromatography; Mass spectometry; Head space method; Solvent extraction method; Gasoline vapor; 鎖状炭化水素; 血中濃度

A reliable method has been developed for analyzing small amounts of fuel components in biological materials, using two simultaneous procedures, that is head space and solvent extraction methods. Gas chromatography/mass spectrometry (GC/MS) was used for qualitative and quantitative determinations.Aliphatic hydrocarbons with carbon numbers of 5 to 8 and aromatics such as benzene, toluene and xylenes were detected in laboratory animals following exposure to gasoline vapor, using the head space method. Aliphatic hydrocarbons, with carbon numbers over 9 as well as aromatics with the carbon number 9 including cumene, mesitylene, pseudocumene and 1,2,3-trimethylbenzene were determined by the solvent extraction method following exposure to kerosene vapor. Both methods were found to be applicable in confirming the cause of human deaths.Since most of the commercially available equimpent for exposure is not economical for use in a small laboratory, we designed a device for generating fuel vapor and can now perform routine experiments, using common tools. the tested solvent, gasoline, is vaporized by warming and is introduced into a series of chambers in which the concentrations can be regulated and stabilized. Vaporized gasoline concentrations are determined quantitatively under stable conditions for at least 60 min. in the case of animal experiments, a positive correlation was observed between the vapor concentration of gasoline and the blood level of each gasoline component. The apparatus proved to be of practical use for estimating the concentrations of gasoline vapor.

建立了一种同时进行顶空萃取和溶剂萃取的生物样品中微量燃料组分的分析方法。采用气相色谱/质谱联用（GC/MS）进行定性和定量分析，采用顶空法测定了实验动物暴露于汽油蒸气后体内碳原子数为5 ~ 8的脂肪烃和苯、甲苯、二甲苯等芳烃的含量。采用溶剂萃取法测定了煤油蒸气中碳原子数大于9的脂肪烃和碳原子数为9的芳烃，包括异丙苯、均三甲苯、偏三甲苯和1，2，3-三甲苯。这两种方法被认为是适用于确认人类死亡的原因。由于大多数商业上可获得的equimpent暴露是不经济的使用在一个小实验室，我们设计了一个装置，用于产生燃料蒸气，现在可以进行常规实验，使用普通的工具。测试溶剂汽油通过加热而蒸发，并被引入到一系列腔室中，在所述腔室中浓度可以被调节和稳定。在稳定条件下至少60 min定量测定汽化汽油浓度。在动物实验中，观察到汽油蒸汽浓度与每种汽油组分的血液水平之间呈正相关。该装置被证明是一种实用的汽油蒸气浓度的估计。

项目成果

Kojiro KIMURA: "A Newly Designed Apparatus for Testing Blood Level of Fuel Vapor" Forensic Science International. 40. 57-61 (1989)

Kojiro KIMURA：“一种新设计的用于测试燃油蒸气血液水平的装置”国际法医科学。