Effect of basic fibroblast growth factor on regional myocardial blood flow of acutely infarcted myocardium

碱性成纤维细胞生长因子对急性梗死心肌局部心肌血流的影响

• 批准号: 06670751
• 负责人: ISHIKAWA Kinji
• 金额: $ 1.22万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670751/
• 关键词: Myocardial infarction; Collateral flow; regional myocardial flow; Basic fibroblast growth factor; Angiogenesis; Non radioactive colored microsphere

This study was designed to clarify whether basic fibroblast growth factor (bFGF) might increase regional myocardial blood flow (Qm) at the acutely infarcted myocardium in experimental animals. Methods : In 22 SD rats, the chest was opened and the left coronary artery was ligated. The chest was closed and bred for 4 weeks. Nonradioactive colored microsphere technique was used to measure Qm. In 11 rats, bFGF was not used while in the other 11 rats, bFGF 100mug was injected into the infarcted myocardium immediately after coronary occlusion. After 4 weeks, the heart was excised for the histological examinations. In 25 dogs, the left anterior descending coronary artery was ligated and bred for 4 weeks. Qm was measured using nonradioactive colored microsphere technique. In 11 dogs, bFGF 300mug was injected into the myocardium just before coronary occlusion, while in the other 14 dogs, bFGF was not given. Results : In rats, bFGF failed to change Qm of the infarcted myocardium as well as border zone and noninfarcted myocardium. Infarct size was not reduced in rats by bFGF.In dogs, Qm in control group and bFGF group were 24(]SY.+-.])4,63(]SY.+-.])10% respectively at the inner layr ; 23(]SY.+-.])5,100(]SY.+-.])20% at the middle layr and 74(]SY.+-.])7,130(]SY.+-.])16% at the outer layr. This study showed thatr bFGF increased Qm at the infarcted myocardium in dogs while bFGF had no effect in rats.Conclusion : Basic FGF had a significant effect to increase Qm at the infarcted myocardium in dogs. This may offer a new method for the treament of ischemic heart disease.

本研究旨在阐明碱性成纤维细胞生长因子（bFGF）是否可能增加实验动物急性梗死心肌的局部心肌血流量（Qm）。方法：22只SD大鼠开胸结扎左冠状动脉。封闭胸腔，饲养4周。采用非放射性有色微球技术测量Qm。其中11只大鼠未使用bFGF，另外11只大鼠在冠状动脉闭塞后立即向梗死心肌注射bFGF 100mug。4周后，切除心脏进行组织学检查。25只犬结扎左冠状动脉前降支，饲养4周。Qm采用非放射性有色微球技术测量。11只狗在冠状动脉闭塞前心肌注射bFGF 300mug，其余14只狗不注射bFGF。结果：bFGF对大鼠梗死心肌及边界区、非梗死心肌的Qm均无明显改变。bFGF没有减少大鼠的梗死面积。对照组和bFGF组Qm分别为24([SY.+-])4、63（[SY.+-]）。])内层分别为10%；23日(SY。+ -))5100 (SY。+ -。]) 20%,中间layr和74 (SY。+ -))7130 (SY。+ -。])外层16%。本研究显示bFGF可增加狗梗死心肌的Qm，而对大鼠无影响。结论：碱性FGF对心肌梗死Qm有明显的提高作用。这可能为缺血性心脏病的治疗提供一种新的方法。

项目成果

K Ishikawa et al: "Basic fibroblast growth factor increased collateral flow to the infarcted myocardium in rats" Europ Heart J. 16-suppl. 171 (1995)

K Ishikawa 等人：“碱性成纤维细胞生长因子增加大鼠梗死心肌的侧支血流”Europ Heart J. 16-suppl。

K Ishikawa et al: "Effect of basic fibroblast growth factor on the regional myocardial blood flow of the acutely infarcted myocardium in rats" J Mol Cell Cardiol. 27-11. A505 (1995)

K Ishikawa 等人：“碱性成纤维细胞生长因子对大鼠急性梗塞心肌局部心肌血流的影响”J Mol Cell Cardiol。

K Ishikawa: "Basic fibroblast growth factor increased collateral flows to the infarcted myocardium in rats(abstract)" European Heart J. 16. 171 (1995)

K Ishikawa：“碱性成纤维细胞生长因子增加大鼠梗塞心肌的侧支血流（摘要）”《欧洲心脏杂志》16. 171 (1995)

K Ishikawa: "Basic fibroblast growth factor increased collateral flow to the infarcted myocardium in rats(abstract)" European Heart J. 16. 171- (1995)

K Ishikawa：“碱性成纤维细胞生长因子增加大鼠梗死心肌的侧支血流（摘要）”《欧洲心脏杂志》16. 171-（1995）

M Miyataka: "Basic fibroblast growth factor failed to augment regional myocardial blood flow to the acutely infarcted myocardium in dogs(abstract)" J Mol Cell Cardiol. 27. A505 (1995)

M Miyataka：“碱性成纤维细胞生长因子未能增加狗急性梗塞心肌的局部心肌血流量（摘要）”J Mol Cell Cardiol。