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Experimental studies on the angiogenesis at the infarcted portion using basic fibroblast growth factor Professor

使用碱性成纤维细胞生长因子对梗塞部位血管生成的实验研究教授

基本信息

批准号：
08670833
负责人：
ISHIKAWA Kinji
金额：
$ 1.47万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670833/
关键词：
basic fibroblast growth factor Angiogenesis Regional myocardial blood flow Acute myocardial infarction Myocardial salvage Myocardial viability Infarct size acute myocardial infarction myocardial blood flow basic fibroblast growth factor

项目摘要

The purpose of this study is to clarify whether basic fibroblast growth factor (bFGF) might increase regional myocardial blood flow (Qm) at the infarcted myocardium and salvages it in the acutely infarcted myocardium in experimental animals.[Methods] In 20 mongrel dogs, 28 SD rats and 37 rabbits, the coronary artey were ligated, bFGF 300 mu g in dogs, 100 mu g in SD rats and 100 mu g in rabbits were injected respectively into the infarcted myocardium and bred for 4 weeks. Qm was measured using nonradioactive colored microsphere and the amount of viable myocardium and extent of fibrosis in the infarcted area were measured histologically.[Results] Qm was increased and viable myocardium was plenty and fibrosis was less at the infarcted area in dogs treated with bFGF.In SD rats, however, bFGF caused no change. In rabbits, the samse effects were observed at the border zone but not at the infarcted area.[Conclusion] The beneficial effects of bFGF at the infarcted area were observed only in dogs. The same effects were seen at the border zone in rabbits but no effect in SD rats. These obervations suggest that the effects of bFGF were not uniform and there are species differences in the effect of bFGF.

本研究旨在探讨碱性成纤维细胞生长因子（bFGF）是否能增加实验动物急性心肌梗死局部心肌血流量（Qm），并挽救急性心肌梗死局部心肌血流量。[方法]杂种犬20只，SD大鼠28只，家兔37只，结扎冠状动脉，分别于犬、SD大鼠和家兔梗死心肌内注射bFGF 300 μ g、100 μ g和100 μ g，饲养4周。用非放射性染色微球测量Qm，组织学测量梗死区存活心肌量和纤维化程度。[结果] bFGF治疗后犬心肌梗死区Qm增加，存活心肌丰富，纤维化程度减轻，而SD大鼠心肌梗死区Qm无明显变化。在家兔中，在边缘区观察到同样的作用，而在梗塞区则没有。[结论] bFGF仅对犬脑梗死有保护作用。在家兔的边缘区观察到相同的效果，但在SD大鼠中没有效果。这些结果提示bFGF的作用并不均匀，其作用存在种属差异。