Basic Fibroblast growth factor improves high energy phosphate metabolism in the ischemic myocardium in rabbits

碱性成纤维细胞生长因子改善兔缺血心肌高能磷酸盐代谢

• 批准号: 11670719
• 负责人: ISHIKAWA Kinji
• 金额: $ 2.05万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670719/
• 关键词: Basic fibroblast growth factor; Angiogenesis; Ischemic myocardial metabolism; Acute myocardial infarction; ^<31>P-NMR; Myocardial salvage; Infarct size; Cardioprotective effect; 局所心筋血流量; 心筋viability

Basic fibroblast growth factor (bFGF) has been shown effective in the treatment of chronic iscnemic myocardium as well as in acute myocardial infarction. The beneficial role to limit infarct size following abrupt coronary occlusion can not be attributable solely to angiogenesis and myocardial salvage independent of angiogenesis or cardioprotective effect of bFGF has been proposed. We hypothesized that bFGF may modify high energy phosphate metabolism in the ischemic myocardium to extend cell survival and prolong myocardial survival. In 17 Japanese white rabbits, the heart was perfused with Langendorff preparation and adenosine triphosphate (ATP), creatine phosphate (CrP) and inorganic phosphate (Pi) were measured every 5 min using ^<31>P-NMR during 10 min basal state, 20 min global ischemia following by 60 min postischemic reperfusion. In 8 of these, bFGF 100 μg was injected in the perfusate 1 min prior to the global ischemia. ATP was higher during postischemic reperfusion in bFGF-treated heart.In conclusion, bFGF was effective to maintain ATP higher in the postischemic myocardium. This effect may contribute to limit infarct size in the acute myocardial infarction.

碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）已被证明在治疗慢性缺血性心肌和急性心肌梗死中有效。已经提出，限制突然冠状动脉闭塞后的梗死面积的有益作用不能仅仅归因于血管生成和独立于血管生成或bFGF的心脏保护作用的心肌挽救。我们推测bFGF可能通过改变缺血心肌的高能磷酸盐代谢来延长细胞存活和心肌存活。在17只日本白色家兔的心脏内灌注Langendorff制剂，在基础状态10 min、全脑缺血20 min和缺血后再灌注60 min期间，每5 min用~ 1H-NMR测定三磷酸腺苷（ATP）、磷酸肌酸（CrP）和无机磷（Pi）<31>。其中8例于缺血前1 min在灌流液中注入bFGF 100 μg。bFGF处理的心脏在缺血后再灌注期间ATP较高。总之，bFGF有效地维持缺血后心肌中较高的ATP。这种作用可能有助于限制急性心肌梗死的梗死范围。

项目成果

宮高 昌, 木村彰男 et al.: "虚血/再潅流心における塩基性線維芽細胞増殖因子の心筋虚血に及ぼす影響"心筋の構造と代謝. 23. 271-277 (2001)

Masaru Miyataka、Akio Kimura 等人：“碱性成纤维细胞生长因子对缺血/再灌注心脏心肌缺血的影响”《心肌结构与代谢》23. 271-277 (2001)。

Masaru Miyataka,Kinji Ishikawa, et al: "Basic Fibroblast Growth Factor Increased Regional Myocardial Blood Flow and Limited Infarct Size of Acute Infarcted Myocardium in Dogs"Angiology. 49. 381-390 (1998)

Masaru Miyataka、Kinji Ishikawa 等人：“碱性成纤维细胞生长因子增加了犬的局部心肌血流量并限制了狗急性梗塞心肌的梗塞面积”血管学。

Masaru Miyataka,Kinji Ishikawa: "Intramyocardial Injection of Basic Fibroblast Growth Factor Increased Regional Myocardial Blood Flow and Salvaged Infarcted Myocardium in Dog"Kluwer Academic Publishuers. 6 (1998)

Masaru Miyataka、Kinji Ishikawa：“心肌内注射碱性成纤维细胞生长因子增加了狗的局部心肌血流量并挽救了梗塞心肌”Kluwer学术出版社。

Kinji Ishikawa: "Effect of Basic Fibroblast Growth Factor on the Regional Myocardial Blood Flow of Acutely Infarcted Myocardium in Experimental Animals"Kluwer Academic Publishers. 8 (1998)

石川欣二：《碱性成纤维细胞生长因子对实验动物急性梗塞心肌局部心肌血流的影响》Kluwer学术出版社。

Takaya Hasegawa,Kinji Ishikawa: "Basic Fibroblast Growth Factor Increases Regional Myoacardial Blood Flow and Salvages Myocardium in the Infarct Border Zone in a Rabbit Model of Acute Myocardial Infarction"Angiology. 50. 487-495 (1999)

Takaya Hasekawa、Kinji Ishikawa：“碱性成纤维细胞生长因子增加急性心肌梗塞兔模型中的局部心肌血流量并挽救梗塞边界区的心肌”血管学。