Studies on the expressin of adhesion molecules on Fibroblats and lenhaytes by cytonagslovirus

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We investigated the adherence of peripheral blood leukocytes to cytomegalovirus (CMV) -infected and uninfected fibroblasts. Adherence of fresh peripheral blood mononuclear cells (PBMC) to CMV-infected fibroblasts was significantly greater than their adherence to uninfected fibroblasts. In leukocyte populations, fresh monocytes and B cells adhered to CMV-infected fibroblasts, whereas fresh T cells and polymorphonuclear leukocytes (PMNL) did not adhere to either CMV-infected or uninfected fibroblasts. When T cells were activated with anti-CD3 antibody, activated T cells demonstrated the adherence and cytotoxicity to both CMV-infected and uninfected fibroblasts. Magnitude of cytotoxicity mediated by adherent activated T lymphocytes was significantly greater that that of unifected fibroblasts. Adherence of PBMC and cytotoxicity mediated by adherent activated Tcells were blocked by treatment of CMV-infected fibroblasts with anti-ICAM-1 antibody and by treatment of leukocytes with anti-LFA-1 antibody. These data suggest that an interaction of ICAM-1 and LFA-1 is responsible for the adherence oc leukocytes and for adherent activated T cell-mediated cytotoxicity against CMV-infected fibroblasts.