Studies on the effect of HSV-1 on apoptosis and cell cycle

HSV-1对细胞凋亡和细胞周期影响的研究

• 批准号: 10670724
• 负责人: ITO Masahiro
• 金额: $ 2.05万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670724/
• 关键词: HSV-1; apoptosis; lymphocytes; 単純ヘルペスウイルス; リンパ球; アポトーシス; 単純ヘルペスウィルス

We investigated apoptosis induced by herpes simplex virus type 1 (HSV-1) in cord blood T lymphocytes by using agarose gel electrophoresis, DNA content analysis and the terminal deoxytransferase (TdT)- mediated dUTP nick end-labeling (TUNEL) method. DNA fragmentation and the hypodiploid fraction in the cell cycle were both increased in HSV-1-infected CD4 and CD8 lymphocytes stimulated with phytohaemagglutinin (PHA) compared to mock- infected lymphocytes. HSV-1 antigen glycoprotein D (gD) and regulatory protein ICP27 were detected in 8-18% of the hypodiploid fraction of PHA-stimulated, HSV-1-infected lymphocytes. Apoptosis was induced not only in HSV-1 antigen-expressing cells but also in cells not expressing detectable viral proteins. Addition of anti-Fas antibody, anti-Fas-ligand antibody or a mixture of both had no effect on HSV-1-induced apoptosis, indicating that the Fas-Fas-ligand pathway did not contribute to HSV-1-induced apoptosis.

采用琼脂糖凝胶电泳、DNA含量分析和末端脱氧转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）法研究单纯疱疹病毒1型（HSV-1）诱导的脐血T淋巴细胞凋亡。与模拟感染的淋巴细胞相比，用植物血凝素（PHA）刺激的HSV-1感染的CD 4和CD 8淋巴细胞的DNA片段化和细胞周期中的亚二倍体分数都增加。HSV-1抗原糖蛋白D（gD）和调节蛋白ICP 27检测PHA刺激，HSV-1感染的淋巴细胞的亚二倍体部分的8-18%。不仅在HSV-1抗原表达细胞中诱导凋亡，而且在不表达可检测病毒蛋白的细胞中也诱导凋亡。添加抗Fas抗体、抗Fas配体抗体或两者的混合物对HSV-1诱导的细胞凋亡没有影响，表明Fas-Fas配体途径对HSV-1诱导的细胞凋亡没有贡献。

项目成果

Ito M.et al.: "Herpes Simplex Virns Typel Indu us spoprosis in Periplorcl BLood Trympls ayfes" Journal of Imfpchons Dineases. 175. 1220-1224 (1997)

Ito M.等人：“Periplorcl BLood Trympls ayfes 中的单纯疱疹病毒 Indu us spoprosis”《Imfpchons Dineases》杂志。

Ito M.et al.: "Apoptosis of cord blood lymphocytes by herpes virus type 1"Journal of Medical Virology. 78. 1971-1975 (1997)

Ito M.等人：“1 型疱疹病毒引起的脐带血淋巴细胞凋亡”医学病毒学杂志。

Itoh M. et al.: "Apoptosis of cord Blood Thymphocytes by Herpes Simplex Virus Type1"Journal of Hemerol Virology. 78. 1971-1975 (1997)

Itoh M.等人：“单纯疱疹病毒1型引起的脐带血胸腺细胞凋亡”《赫默罗病毒学杂志》。

ITO M, et al.: "Changes in intracellular cytohome levels in new born and adults lymphocytes induced by HSV-1"Journal of Medical Virology. 56. 145-150 (1998)

ITO M 等人：“HSV-1 诱导的新生儿和成人淋巴细胞中细胞内细胞组水平的变化”医学病毒学杂志。

Itoh M. et al.: "Changes in Intracellular Cytohine Le*elsin Nenburn and adult Lymphocytes Induced by HSV-1"Journal of Medical Virology. 56. 145-150 (1998)

Itoh M. 等人：“HSV-1 诱导的细胞内细胞因子 Le*elsin Nenburn 和成体淋巴细胞的变化”医学病毒学杂志。