DISEASE-ACTIVITY MARKERS IN EPSTEIN-BARR VIRUS INFECTION AND ESTABLISHMENT OF CELL-LINE POSITIVE FOR EB VIRUS.

Epstein-Barr 病毒感染中的疾病活动标记和 EB 病毒阳性细胞系的建立。

• 批准号: 06670814
• 负责人: YOKOTA Shumpei
• 金额: $ 1.34万
• 依托单位: YOKOHAMA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670814/
• 关键词: EPSTEIN-BARR VIRUS; CYTOKINES; DISEASE-ACTIVITY; FERRITIN; CYCLOSPORIN A; TARGETTING THERAPY; リポ化ステロイド; サイクロスポリンA; 慢性活動性EBウイルス感染症

Firstly we established the PCR detection system for EB virus using primers originated from IR1 gene sequences. The detection system was useful, and detected EBV genome in mononuclear cells in peripheral blood, inflammatory cells in pleural effusion, and bone marrow cells. The activated macrophages and lymphocytes were the populations of the exudative cells. The disease activity of chronic active EBV infection and virus-associated hemophagocytic syndrome was monitered well by AST,ALT,LDH,fibrinogen-fibrin degrades (FDP-E,D dimer), and serum ferritin. Cytokines originated from activated macrophages and T cells were abnormally high in these diseases, and the regulatory imbalances of these cytokines may be contributed to generalized vasculitis. Therapy was initiated to stabilize macrophages and T cells by administratiog lipo-dezamethasone and cyclosporin A.

本研究首先利用EB病毒IR 1基因序列设计引物，建立了EB病毒PCR检测体系。该检测系统可用于检测外周血单个核细胞、胸腔积液炎性细胞和骨髓细胞中的EBV基因组。渗出细胞主要为活化的巨噬细胞和淋巴细胞。血清AST、ALT、LDH、纤维蛋白原-纤维蛋白降解产物（FDP-E、D-二聚体）和血清铁蛋白能很好地监测慢性活动性EBV感染和病毒相关噬血细胞综合征的病情活动。在这些疾病中，来自活化的巨噬细胞和T细胞的细胞因子异常升高，这些细胞因子的调节失衡可能导致全身性血管炎。通过给予脂质体和环孢菌素A开始治疗以稳定巨噬细胞和T细胞。

项目成果

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Yokota S. et al: "Superinfection with hepatitis C virus sub typesin frequently" European Journal of Pediatries. 614-616 (1994)

Yokota S. 等人：“丙型肝炎病毒亚型频繁重复感染”《欧洲儿科杂志》。