权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of 1pr-dnT Cell Function in Sle-Prone Mouse, MRL/1pr.

易患 Sle 小鼠的 1pr-dnT 细胞功能分析，MRL/1pr。

基本信息

批准号：
63570449
负责人：
YOKOTA Shumpei
金额：
$ 1.34万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570449/
关键词：
MRL 1pr SLE-prone mouse stem cell antigen premature T cell モデル・マウス MRL@lprマウス TGFβ T幹細胞単クロ-ン抗体 T細胞分化 c-myb癌遺伝子 SLE自然発症マウス増殖因子

项目摘要

We investigated the expression of T-cell surface antigens and the functional characteristics specific for stem cells or early remature T-cells in MRL/1pr mouse in the present study.As previously reported, MRL/1pr lymph node cells was Lyt-2 negative, L3T4 negative which were conventional surface antigens of cytotoxic and helper T-cell subsets, respectively. Using IgM type cytotoxic antibodies against LyT-2 and L3T4 with rabbit complement, Lyt-2 (-), L3T4 (-) double negative T-cells were purified, and subjected to the further experiments. The 1pr-double negative T-cells were found that they were positive for Pgp-1 and B220 antigens, which were markers of premature T-cells. We then developed monoclonal antibody which recognize stem T-cells or very immature T-cells. These monoclonal antibodies, designated as Sca-1 and Sca-2, were used for the detection of the surface antigens of 1pr-double negative cells. Our findings indicated that 1pr-double negative T-cells expressed Sca-1 but not Sca-2, suggesting that 1pr-double negative T-cells were on the T-cell lineage, and they were really premature state. In the next experiments we investigated the growth factors which might be the products of 1pr-double negative cells. The purified 1pr-double negative T-cells were cultured in 96-well plates in RPMI 1640 media. The supernatants were analyzed after purification by gel-filtration technique. We found that the supernatant contained growth factors which had function like tumor growth factor beta. Further study will be needed to clarify the function of 1pr-double negative T-cells.

本研究观察了MRL/1 pr小鼠淋巴结细胞的T细胞表面抗原的表达及其对干细胞和早期再成熟T细胞的特异性功能特性，发现MRL/1 pr小鼠淋巴结细胞的Lyt-2和L3 T4均为阴性，而Lyt-2和L3 T4分别为细胞毒性T细胞和辅助性T细胞的常规表面抗原。使用抗LyT-2和L3 T4的IgM型细胞毒性抗体与兔补体，纯化Lyt-2（-）、L3 T4（-）双阴性T细胞，并进行进一步的实验。发现1 pr-双阴性T细胞对Pgp-1和B220抗原呈阳性，这是未成熟T细胞的标志物。然后，我们开发了识别干T细胞或非常不成熟的T细胞的单克隆抗体。这些单克隆抗体，命名为Sca-1和Sca-2，用于检测1 pr-双阴性细胞的表面抗原。结果表明，1 pr-双阴性T细胞表达Sca-1，不表达Sca-2，提示1 pr-双阴性T细胞属于T细胞谱系，处于真正的早熟状态。在接下来的实验中，我们研究了可能是1 pr-双阴性细胞产物的生长因子。将纯化的1 pr-双阴性T细胞在96孔板中在RPMI 1640培养基中培养。通过凝胶过滤技术纯化后分析上清液。我们发现上清中含有具有肿瘤生长因子β功能的生长因子。需要进一步研究以阐明1 pr-双阴性T细胞的功能。