Molleculor Biological Analysis of the role of B71BB1 in contact dermatitis

B71BB1 在接触性皮炎中作用的分子生物学分析

• 批准号: 06670855
• 负责人: YOKOZEKI Hiroo
• 金额: $ 1.28万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670855/
• 关键词: Contact Dermatitis; T cell Activation; Costimulatory molecnles; CD80 (B7-1); CD86 (B7-2); Langerhans cell; T cell; T細胞; CD80抗原; CD86抗原

Recently, an alternative CD28 ligand, CD86 (B70/B7-2) has been identified on activated B cells. It has been also found that CD86 may play an important role for costimulation of T cells in a primary immune response (Nature : 366,76-79,1993). These finding led us to determine whether CD80 and CD86 antigens express on Langerhans cells (LC) and costimulatory molecules transduced signals play a role in T cell activation in contact hypersensitivity.We first examined CD80, CD86 expression on EC of organ cultured human skin, by staining with the monoclonal anti CD80 (L307), anti CD86Ab (IT2). Keratinocyte (KC) expressed little CD80, CD86, but both CD80 and CD86 molecules on Langerhans cell (LC) have been expressed extensively strongly after culturing. Both CD86 and CD 86 molecules have been detected in contact ddermatitis, atopic dermatitis and psoriasis. In these cases, expression of CD86 was predominantly induced. To determine whether IT2 react with 70KD glycoprotein on LC,immunoblotting method was conducted. IT2 was reacted mainly with the 70KD peptide of LC.We also examined the effect of mAb anti-CD80 or mAb anti-CD86 on the capacity of cultured EC to stimulate proliferation of allogeneic peripheral blood mononuclear cellc (PBMC). Both anti CD80 mAb and anti CD86 mAb efficiently inhibited the mixed epidermal cell-lymphocyte reaction.These data indicate that CD86 (B70/B7-2) antigen expressed on LC may play an important role for costimulation of T cells in a primary immune response ; contact dermatitis.

最近，在活化的B细胞上发现了另一种CD28配体CD86 （B70/B7-2）。研究还发现，CD86可能在原发性免疫应答中对T细胞的共刺激中发挥重要作用（Nature: 366,76-79,1993）。这些发现使我们确定CD80和CD86抗原是否在朗格汉斯细胞（LC）上表达，以及共刺激分子转导信号是否在接触性超敏反应中的T细胞激活中起作用。我们首先用抗CD80 （L307）、抗CD86Ab （IT2）单克隆染色法检测了CD80、CD86在体外培养人皮肤EC上的表达。角化细胞（KC）表达CD80和CD86较少，但在朗格汉斯细胞（LC）上CD80和CD86分子经过培养后广泛而强烈地表达。CD86和CD86分子在接触性皮炎、特应性皮炎和牛皮癣中均有检测到。在这些病例中，CD86的表达主要是诱导的。采用免疫印迹法测定LC上IT2是否与70KD糖蛋白发生反应。IT2主要与LC的70KD肽反应。我们还检测了mAb抗cd80或mAb抗cd86对培养EC刺激异体外周血单核细胞（PBMC）增殖能力的影响。抗CD80单抗和抗CD86单抗均能有效抑制混合表皮细胞-淋巴细胞反应。这些数据表明，CD86 （B70/B7-2）抗原在LC上表达可能在原发性免疫应答中对T细胞共刺激起重要作用；接触性皮炎。

项目成果

横関博雄: "アトピー性皮膚炎Q&A(西岡 清 編)" 医薬ジャーナル社, 141 (1995)

横关博夫：“特应性皮炎问答（西冈清编辑）” Iyaku Journalsha，141（1995）

Hiroo Yokozeki: "Experimental study for the development of an in vitro Test for contact allergens." Int.Arch Allergy Immnol. 106. 394-400 (1995)

Hiroo Yokozeki：“开发接触性过敏原体外测试的实验研究。”

Hiroo Yokozeki: "Functional CD86(B7-2) on cultured human Langerhas cells" J.Invest.Dermctol.(in press). (1996)

Hiroo Yokozeki：“培养的人 Langerhas 细胞上的功能性 CD86(B7-2)”J.Invest.Dermctol.（出版中）。

Ichiro Katayama: "Induction and characterization of mast cell colonies by culture supernatant of retinoid acid-treated mouse keratinocytes" Int.Arch.Allerg.Immunol. 100. 328-332 (1993)

Ichiro Katayama：“通过视黄酸处理的小鼠角质形成细胞的培养上清液诱导和表征肥大细胞集落”Int.Arch.Allerg.Immunol。

横関博雄: "成人型アトピー性皮膚炎の重症度点数化の検討" 日本皮膚科学会誌雑誌. 105. 707-712 (1995)

Hiroo Yokoseki：“成人发病特应性皮炎严重程度评分的研究”日本皮肤病学会杂志 105. 707-712 (1995)。