Functional Characteristics in the Embryo of Mouse Model of Transposition of the Great Arteries
大动脉转位小鼠胚胎模型的功能特征
基本信息
- 批准号:06671172
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We had been trying to make a good model of transposition of the great arteries (TGA) in the mouse by treating pregnant dams by all-trans retinoic acid, and finally established the model in which TGA was induced in 90% of embryos by giving the drug with a dose of 70mg/kg at 8.5 days of gestation. Using this model, we have found that hypo-dysplasia of proximal outflow swelling causes failure of the normal ventriculo-arterial ralation and leads to parallel connection of the ventricles to the great arteries, i.e.the right ventricle (RV) to the aorta (Ao) and the left ventricle (LV) to the pulmonary artery (PA). We have then considered that it is important to find the role of hemodynamics in its morphogenesis, and established the technique in which the mammalian embryo is observed in situ under a microscope and action of the heart is recorded with a high speed video camera while it is still alive and viable. Using this method, it was found that there was two pattaerns of flow through outflow tract of the primitive ventricle in TGA model embryos ; one is that flow from RV to Ao was parallel to and separated from it from LV to PA ; the other was that flow from RV goes into both Ao and PA.The latter likely destined to TGA associated with malalign type ventricular septal defect, or double outlet right ventricle in an extreme case. Analysing the ventricular size and function, RV was smaller and lower in ejection fraction in TGA embryos than in controls, suggestings some possible role of hemodynamics in pathogenesis of TGA.
本实验采用全反式维甲酸(all-trans-retinoic acid,ATRA)对孕鼠进行处理,试图建立一种较好的小鼠大动脉转位(translocation of the great arteries,TGA)模型,并在孕8.5天给予ATRA 70 mg/kg,成功地建立了90%胚胎发生TGA的模型。利用该模型,我们发现近端流出道肿胀发育不良导致正常心室-动脉关系的失败,并导致心室与大动脉的平行连接,即右心室(RV)与主动脉(Ao)和左心室(LV)与肺动脉(PA)。我们认为,重要的是要找到它的形态发生的血液动力学的作用,并建立了技术,其中哺乳动物胚胎在显微镜下原位观察和心脏的活动,而它仍然活着和可行的高速摄像机记录。结果表明,TGA模型胚胎原始心室流出道存在两种血流模式:一种是RV至Ao的血流与LV至PA的血流平行分离;另一种是右室流出的血流同时进入主动脉和肺动脉,后者可能导致TGA合并排列不齐型室间隔缺损,或者在极端情况下是右心室双出口。分析TGA胚胎的心室大小和功能,发现TGA胚胎的RV比对照组小,射血分数低,提示血流动力学在TGA发病中可能起一定作用。
项目成果
期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.MORISHIMA et al: "Visceroatrial heterotaxy syndrome induced by mcternal hyperthermia in the rat" Cardiol Young. 5(印刷中). (1995)
M.MORISHIMA 等人:“大鼠子宫热疗引起的内脏房异位综合征”Cardiol Young 5(出版中)。
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- 影响因子:0
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- 通讯作者:
Nakazawa M et al: Functional characteristic of the embryonic heart. in Clark EB,Markwald RP,and Takao A (ed) Development Mechanisms of Heart Diseases.Futura Publ, Mount Kisco, NY, 435 (1995)
Nakazawa M 等人:胚胎心脏的功能特征。
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- 影响因子:0
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Nakazawa M et al:"Developmental Mechanisms of Heart Disease" Clark EB,Markwald RR,Takao A,(ed),Futura Publ,Mount Kisco,NY,679 (1995)
Nakazawa M 等人:“心脏病的发展机制”Clark EB,Markwald RR,Takao A,(ed),Futura Publ,Mount Kisco,NY,679 (1995)
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- 影响因子:0
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Yasui H,Nakazawa M,Morishima M,Miyagawa-Tomita S,Momma K: "Morphological observation on the pathogenetic process of transposition of the great arteries induced bu retinoic acid in mice." Circulation. 91. 2478-2486 (1995)
Yasui H,Nakazawa M,Morishima M,Miyakawa-Tomita S,Momma K:“小鼠大动脉转位诱导的布维A酸发病过程的形态学观察”。
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- 影响因子:0
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Miyagawa-Tomita S,Nakazawa M: On the mechanisms of morphogenesis of cardio vascular anomalies. In Nakazawa M (ed) Congenital and Aquired Heart Diseases in Childhood. Nankoudo, Tokyo, (1995)
Miyakawa-Tomita S,Nakazawa M:心血管异常形态发生的机制。
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NAKAZAWA Makoto其他文献
NAKAZAWA Makoto的其他文献
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{{ truncateString('NAKAZAWA Makoto', 18)}}的其他基金
Analysis of elementary school student's thinking process at programming learning and design of educational support system based on it
小学生编程学习思维过程分析及基于此的教育支持系统设计
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17K01101 - 财政年份:2017
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$ 1.34万 - 项目类别:
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Corporate Marketing Strategies among Nonprofit Sports Organizations
非营利体育组织的企业营销策略
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15500427 - 财政年份:2003
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis for vasculogenesis using genetic engineering mice
使用基因工程小鼠进行血管生成分析
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15390335 - 财政年份:2003
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$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanism and Hemodynamic Function in the Outflow Tract of Heart
心脏流出道的分子机制和血流动力学功能
- 批准号:
12470172 - 财政年份:2000
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Retinoic acid receptors in mouse hearts during development
小鼠心脏发育过程中的视黄酸受体
- 批准号:
09671199 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Hemodynamic effects of teratogenic agents on cardiovasculat function during early developmental stage of morphogenesis
致畸剂对形态发生早期发育阶段心血管功能的血流动力学影响
- 批准号:
61570474 - 财政年份:1986
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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