The Study of Embryo Maturation Factor Derived from Endometrial Stromal Cells

子宫内膜基质细胞胚胎成熟因子的研究

• 批准号: 06671674
• 负责人: UMESAKI Naohiko
• 金额: $ 1.34万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671674/
• 关键词: EMBRYONIC DEVELOPMENT; ENDOMERTRIUM; ENDOMETRIAL STROMAL CELL; ENDOMETRIOSIS; PERITONEAL FLUID; Endometrial stromal cell; 子宮内膜間質細胞; 受精卵成熟促進因子; 子宮内膜症; 体外受精・胚移植

Embroys implant at endometrium after development in the tube and intra-uterine cavity. So endometrium may play a role of enhancement of embryonic development. In this study, we evaluated the action of endometrium on early embryonic development by using co-culture system with endometrial stromal cells (ESCs) and mice embryos. ESCs were obtained from patients with leiomyoma (control) or endometriosis (EM). ESCs from control increased the embryonic development in comparison with EMS.Peritoneal fluid (PF) from EM suppressed the embryonic development in the co-culture system. Next the influence of serum from EM patients on embryonic development was evaluated. This suppressed the embryonic development. Further, culture of embryo in the culture medium with erythrocytes enhanced the embryonic development. In this studies, co-culture with SMC from control or erytrocytes in culture medium enhanced the embryonic developments and co-culture with stromal cells from EMS and PF or serum from EM suppresed th embryonic development. Now we are studying about enhancing factor from endometrium and suppresing factor PF or serum from EM on embryonic divelopment.

胚胎植入子宫内膜后，在管内和宫腔内发育。因此，子宫内膜可能具有促进胚胎发育的作用。本研究采用子宫内膜间质细胞(ESCs)与小鼠胚胎共培养体系，探讨子宫内膜在早期胚胎发育中的作用。胚胎干细胞取自子宫肌瘤(对照组)或子宫内膜异位症(EM)患者。与EMS相比，来自对照的ESCs促进了胚胎的发育，而来自EM的腹水(PF)抑制了共培养体系中的胚胎发育。其次，评估EM患者血清对胚胎发育的影响。这抑制了胚胎的发育。此外，在含红细胞的培养液中培养胚胎可促进胚胎发育。在本研究中，与对照组的SMC或培养液中的红细胞共培养促进胚胎发育，与EMS和PF的基质细胞或EM的血清共培养抑制TH胚胎的发育。目前，我们正在研究子宫内膜促进因子和抑制子宫内膜因子以及血清对胚胎发育的影响。

项目成果

Nakamura H et al: "Immunohistochemical and ultrastructual stydy of hunan NK Cells in early pregnancy" Japas society of Immunological Reproduction(JSIR). 59-60 (1995)

Nakamura H等人：“妊娠早期湖南NK细胞的免疫组织化学和超微结构研究”日本免疫生殖学会（JSIR）。

Umesaki N et al: "Immunological aspect of progression of endometriosis and iufertiliy associated endometriosis" Proc.XV Asia-Oceanic Congress of OB l GYN. 247-52 (1995)

Umesaki N 等人：“子宫内膜异位症和生育相关子宫内膜异位症进展的免疫学方面”Proc.XV 亚洲-大洋洲妇产科大会。

Matsuoka I et al: "Impact of erythrocytes on mouse embryonal development in vitro" FEBS Letters. 371. 114-117 (1995)

Matsuoka I 等人：“红细胞对体外小鼠胚胎发育的影响”FEBS Letters。

Matsuoka I et al: "Impact of erythrocytes on mouse embryonal Jevelopment in vitro" FEBS letters. 371. 297-299 (1995)

Matsuoka I 等人：“红细胞对体外小鼠胚胎发育的影响”FEBS 信件。

古久保ますみ他: "子宮内膜症における不妊症発症機序の検討" エンドメトリオーシス研究会誌. 16. 60-65 (1995)

Masumi Kokubo 等：“子宫内膜异位症不孕机制的检查”子宫内膜异位症研究组杂志 16. 60-65 (1995)。