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Mechanisms of endometrial tissue reconstitution and basic studies on establishment of endometrial tissue regeneration therapy

子宫内膜组织重建机制及子宫内膜组织再生疗法建立的基础研究

基本信息

批准号：
15591773
负责人：
UMESAKI Naohiko
金额：
$ 2.24万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591773/
关键词：
endometrium stem cell endometriosis adenomyosis growth factor cell differentiation 子宮内膜間質細胞

项目摘要

(1)Characterization of human endometrial stem-like cells : In the present study, cellular heterogeneity and functional subpopulations of human endometrial cells were identified. Results of the study are in preparation for submission.(2)In vitro cell proliferation and differentiation of human endometrial stem-like cells : We have discovered at least 2 types of novel differentiation pathways of human endometrial cells. And we examined in vitro differentiation of the sorted functional cell populations from endometrial cells in order to investigate make clear cellular relationships among the populations. The results are prepared for submission.(3)Re-constitution of murine endometrial tissue in vivo using green mouse-derived endometrial stem-like cells : We have been investigating in vivo re-constitution of murine endometrium in wild mouse with the sorted green mouse cells but have not completed it yet. These experiments will be continued even though the project period is over.(4)Endometrial re-generation in vivo in murine uterus with human endometrial stem-like cells : We have already proven that re-constitution of human endometrial tissue in vivo in mouse needs several cellular subpopulations but not one cell population. Moreover, we have investigated various bioactive substances that regulate re-constitution in vitro and re-generation of human endometrium in murine uteri in vivo. The results are prepared for submission.(5)Identification of specific antigens on human endometrial stem-like cells : We have established more than 5000 of hybridomas that produce anti-endometrial stem-like cells in order to sort the stem-like cells from human endometrial tissue and try to re-constitute human endometrial tissue in SCID or nude mouse uterus. However, we have not found any useful antibody to apply for further experiments yet.

(1)人子宫内膜干细胞的表征：在本研究中，确定了人子宫内膜细胞的细胞异质性和功能亚群。研究结果正准备提交。(2)人子宫内膜干细胞的体外细胞增殖和分化：我们发现了至少2种新的人子宫内膜细胞分化途径。并对子宫内膜细胞的功能细胞群的体外分化进行了研究，以明确这些细胞群之间的细胞关系。结果已准备好提交。(3)利用绿色小鼠来源的子宫内膜干细胞在体内重建小鼠子宫内膜组织：我们正在研究利用筛选好的绿色小鼠细胞在野生小鼠体内重建小鼠子宫内膜，但尚未完成。这些实验将在项目结束后继续进行。(4)利用人子宫内膜干细胞在小鼠子宫内再生子宫内膜：我们已经证明，在小鼠体内重建人子宫内膜组织需要几个细胞亚群，而不是一个细胞群。此外，我们还研究了多种调节人子宫内膜体外重构和小鼠子宫内再生的生物活性物质。结果已准备好提交。(5)人子宫内膜干细胞特异性抗原的鉴定：我们建立了5000多个产生抗子宫内膜干细胞的杂杂瘤，从人子宫内膜组织中分选干细胞，并尝试在SCID或裸鼠子宫中重建人子宫内膜组织。但是，我们还没有发现任何有用的抗体来申请进一步的实验。