Quantitative assay of embryo receptibility in the human endometrial tissue

人类子宫内膜组织中胚胎容受性的定量测定

• 批准号: 10671566
• 负责人: UMESAKI Naohiko
• 金额: $ 2.37万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671566/
• 关键词: implantation; decidualization; endometrial stromal cells; laminin; cytokine; 脱落膜; 子宮内膜

(1) Establishment of in vitro decidualization activity assay of human endometrial stromal cells.We have established a novel in vitro decidualization activity assay system of human endometrial stromal cells by which effects of various bioactive substances on endometrial embryo receptobility can be evaluated individually. By using this assay, for example, effects of cytokines and extracellular matrices on human endometrial decidualization were analyzed. lL-1 and laminin inhibit human endometrial stromal differentiation additively. EGF inhibits decidualization while bestatin, an aminopeptidase-N inhibitor, enhances it. We have also examined the effects of M-CSF, lL-4, lL-6, lL-8, lL-11, Oncostatin-M, LlF. Danazol, and so on.During the development of this study, we have found two new decidualization markers which are regulated by the different mechanisms from regulatory ones of decidual prolactin. We are now investigating the regulation system during decidualization.(2) Antigenic changes of the human endometrial stromal cells during decidualizationIn order to clarify the differentiation processes of the decidualization and functional cellular subpopulations in the human endometrial stromal cells, we have been tried to establish novel anti-stromal cell antibodies to discriminate each cellular subpopulation of the human endometrial stromal cells. More than three thousands of mouse hybridoma were established and screened. We have found several useful antibodies for further studies.

(1)人子宫内膜间质细胞体外蜕膜化活性检测方法的建立我们建立了一种新的人子宫内膜间质细胞体外蜕膜化活性检测系统，可以单独评价各种生物活性物质对子宫内膜胚胎容受性的影响。例如，通过使用该测定，分析了细胞因子和细胞外基质对人子宫内膜蜕膜化的影响。IL-1和层粘连蛋白相加抑制人子宫内膜间质分化。EGF抑制蜕膜化，而氨基肽酶-N抑制剂bestatin增强蜕膜化，我们还检测了M-CSF、IL-4、IL-6、IL-8、IL-11、Oncostatin-M、LIF的作用。在本研究的发展过程中，我们发现了两个新的蜕膜化标志物，它们受与蜕膜催乳素不同的调节机制的调节。我们正在研究蜕膜化过程中的调节系统。(2)人子宫内膜间质细胞在蜕膜化过程中的抗原变化为了阐明人子宫内膜间质细胞的蜕膜化过程和功能细胞亚群的分化，我们尝试建立新型的抗间质细胞抗体来区分人子宫内膜间质细胞的各个细胞亚群。建立和筛选了3000多株杂交瘤细胞。我们已经发现了几个有用的抗体，为进一步的研究。

项目成果

Mizuno K. Tanaka T, et al.: "Inhibition of cAMP-mediated decidualization in human endometrial stromal cells by IL-1β and lamnin"Hormone and Metabolic Research. 31. 307-310 (1999)

Mizuno K. Tanaka T 等人：“IL-1β 和层粘连蛋白对人子宫内膜基质细胞中 cAMP 介导的蜕膜化的抑制”激素和代谢研究 31. 307-310 (1999)。

Tanaka T, Mizuno K, Miyama M, Chang L, Chen H, Ohtaki S, Umesaki N, Ogita S: "Enhanced Fas/CD95-mediated apoptosis by epidermal growth factor in human endometrial epithelial cells"Eur J Obstet Gynecol Reprod.Biol. 86. 189-194 (1999)

Tanaka T、Mizuno K、Miyama M、Chang L、Chen H、Ohtaki S、Umesaki N、Ogita S：“人子宫内膜上皮细胞中表皮生长因子增强 Fas/CD95 介导的细胞凋亡”Eur J Obstet Gynecol Reprod.Biol。

Tanaka T, Umesaki N, et al.: "Enhancement of apoptotic susceptibility by interleukin-1 in human endometrial epithelial cells"Gynecological Endscutinology. 12. 315-319 (1998)

Tanaka T、Umesaki N 等人：“人子宫内膜上皮细胞中白细胞介素 1 增强细胞凋亡的易感性”妇科 Endscutinology。

Tanaka T,Umesaki N,: "Danazol effects on human endometrial cells in vitro" Clinical and Experimental Obstetrics & Gynecology.26 印刷中. (1999)

Tanaka T，Umesaki N，：“达那唑对体外人类子宫内膜细胞的影响”临床和实验妇产科.26 出版（1999 年）。

Tanaka T, Umesaki N, Chen H, Mizuno K, Ogita S: "Danazol effects on human endometrial cells in vitro"Clin.Exp.Obst.&Gyn. 26. 67-70 (1999)

Tanaka T、Umesaki N、Chen H、Mizuno K、Ogita S：“达那唑对体外人类子宫内膜细胞的影响”Clin.Exp.Obst。