权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The role of immunology in the maintenance of pregnancy and appearance of abnormal pregnancy-study of local immunity at the feto-maternal interface

免疫学在妊娠维持和异常妊娠出现中的作用——母胎界面局部免疫研究

基本信息

批准号：
60570790
负责人：
UMESAKI Naohiko
金额：
$ 1.22万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

This study was designed to clarify the immunological mechanism at the feto-maternal interface to maintain pregnancy. Initially, we studied the immune system of lymph node draining uterus(DLN), namely the obturator lymph node, to determine whether maternal immune system responded to fetal antigens at maternal-fetal frontier. DLN was found to be significantly enlarged and T-cells, especially Ia positive T-cells were found to be increased in the immunohistological study. Mitogen response of mononuclear cells in DLN(DLN,MNC.) was also increased. Response of DLM, MNC. to cord blood MNC was higher than response of peripheral blood MNC to it. Suppressive activity of DLN, MNC. was not found. NK activity of it was enhanced. These finding s showed that the mother recognized the fetus as an allograf t and actively responded to it. Mother seemed to be try killing it. Therefore, a mechanism for suppression of the maternal immune response to the fetus was needed to maintain the fetus. Furthermore, the immunological role of decidua which lies between the fetus and mother in pregnancy was studied. Decidual tissue was obtained by miscarriage at first trimester. Single cell suspensions of decidua were obtained by the treatment with collagenase and DNAse. Decidual cells inhibited the mixed lymphocyte reaction (MLR). The supernatant from decidual cells culture (DC.sup.) also inhibited it. One of suppressive factor of DC.sup. was found to be PG. The mechanism of suppression was considered to be due to induction of suppressor cell.In conclusion, the mechanism for maintaining the fetus througout pregnancy, might be that the decidual cell suppresses the maternal immune response to the fetus, even though the mother recognizes the fetus as an allograft.

本研究旨在阐明胎儿-母体界面维持妊娠的免疫机制。最初，我们研究了子宫引流淋巴结（DLN）的免疫系统，即闭孔淋巴结，以确定母体免疫系统是否对母胎边界的胎儿抗原做出反应。免疫组织学研究发现DLN明显增大，T细胞尤其是Ia阳性T细胞增多。 DLN（DLN，MNC.）中单核细胞的有丝分裂原反应也增加。 DLM、跨国公司的回应。对脐带血MNC的反应高于外周血MNC对其的反应。 DLN、MNC 的抑制活动。没有找到。其NK活性增强。这些发现表明，母亲认识到胎儿是同种异体移植物，并积极回应。母亲似乎想杀死它。因此，需要一种抑制母体对胎儿的免疫反应的机制来维持胎儿。此外，还研究了妊娠期间胎儿和母亲之间的蜕膜的免疫作用。蜕膜组织是通过妊娠早期流产获得的。通过胶原酶和DNA酶处理获得蜕膜的单细胞悬液。蜕膜细胞抑制混合淋巴细胞反应（MLR）。来自蜕膜细胞培养物(DC.sup.)的上清液也抑制它。 DC.sup的抑制因素之一。被发现是PG。抑制机制被认为是由于抑制细胞的诱导。总之，在整个妊娠过程中维持胎儿的机制可能是蜕膜细胞抑制母体对胎儿的免疫反应，即使母亲将胎儿视为同种异体移植物。