Morphological and functional studies on NO producing neurons in the brainstem involved in pral sensori-motor functions

脑干中参与感觉运动功能的 NO 产生神经元的形态学和功能研究

基本信息

批准号：
06671809
负责人：
TAKEMURA Motohide
金额：
$ 1.34万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

NADPH-diaphorase (NADPH-d) histochemical staining demonstrated a distinct neural group in the lower brainstem of rats. The NADPH-d stain revealed a Golgi-like network in the dorsomedial spinal trigeminal nucleus oralis (Vo) and rostrolateral solitary tract nucleus (Sn). Another neural group was stained in the paratrigeminal nucleus (paraV), gap region between spinal trigerninal nuclei interpolaris (Vi) and caudalis (Vc), superficial zone of Vc, and much less in the magnocellular zone of Vc. The distribution of NADPH-d-positive neurons in Vo and Sn overlapped mostly with the central terminal area labeled transganglionically with WGA-HRP from nerves innervating the intraoral structures. The distribution of NADPH-d-posibve neurons in Vi and Vc overlapped with the central terminal area from nerves innervating intra- and extra-oral structures. The number of labeled neurons in Vo/Sn decreased one to two weeks after transection of nerves innervating intraoral structures. These results show that NADPH-d-positive neurons in the lower brainstem could be regulated trans-synaptically by primary afferents from the intraoral structures. On embryonic day 12 (EI2), no neurons in the brain were stained for NADPH-d, while blood vessels were stained. On E15, labeled neurons appeared in Vo and Sn. On postnatal day 4 (P4), labeling appeared in the magnocellular zone of Vc. On P10, labeling appeared in paraV and the superficial zone of Vc. After the first expression of NADPH-d in each nuclei, the labeling increased or maintained to the adulthood. The very early appearance of NADPH-d labeling in Vo and Sn, which precedes the appearance of NADPH-d elsewhere in the brainstem, suggests that the nitric oxide/cyclic guanosine monophosphate system has an important role for primitive orofacial sensorimotor reflexive functions.

NADPH-肌酶（NADPH-D）组织化学染色表现出大鼠下部脑干的独特神经群。 NADPH-D染色显示在脊髓三叉神经核（VO）和下外侧单型核（SN）中揭示了高尔基体的网络。另一个神经群在副神经核（PARAV），脊柱三角核之间的间隙区域染色，在脊柱三角核之间（VI）和caudalis（VC），VC的表面区域，在VC的大量区域中较少。 NADPH-D阳性神经元在VO和SN中的分布主要与中央末端区域重叠，该区域与神经内神经的WGA-HRP经过跨环状区域，这些神经支配了口腔内结构。 VI和VC中NADPH-D-D-POSIBVE神经元的分布与中央末端区域与神经内部和外部外结构的神经重叠。神经支配口腔内结构的神经横断后，VO/SN中标记的神经元的数量减少了一到两周。这些结果表明，下部脑干中的NADPH-D阳性神经元可以由口腔内结构的初级传入来调节。在胚胎第12天（EI2）中，对NADPH-D的脑部没有染色的神经元，而血管被染色。在E15上，标记的神经元出现在VO和SN中。在产后第4天（P4），标记出现在VC的大细胞区域中。在P10上，标签出现在Parav和VC的表面区域。在每个核中NADPH-D的第一次表达后，标记增加或维持到成年。 NADPH-D标记在VO和SN中的早期出现，该标记在脑干其他地方的出现之前表明，一氧化氮/环状鸟苷单磷酸盐系统对原始类种族歧视的反射反射功能具有重要作用。