Morpho-functional study of trigeminal neurons in the brain stem that are involved in oro-facial sensori-motor function.

作为DNA结合蛋白的一员，c-Fos在电刺激三叉神经节后在三叉神经感觉核的神经元中上调。阳性神经元呈核特异性分布，表达强度与刺激强度有关。在尾侧亚核（SpVc），标记神经元主要分布在背内侧腹外侧轴的浅层。在口侧亚核，c-Fos阳性神经元仅出现在背内侧亚核。低强度刺激（0.1 mA），激活A-β纤维，和高强度刺激（1.0 mA），激活C-纤维诱导的c-Fos的表达，不同的调节预管理的GABAA和GABAB受体激动剂和拮抗剂。三叉神经感觉核内GABA能的节段性和脊髓上强度依赖性调节是复杂的。鞘内注射c-fos反义寡核苷酸 ...更多信息 降低佐剂诱导的cFos表达和延长的热痛觉过敏，但不正常的伤害性感受。因此，c-Fos表达后伤害性感受参与维持和/或发展的痛觉过敏。大脑皮层的躯体感觉区域的电刺激被应用于许多患有顽固性疼痛的患者，具有良好至极好的疼痛缓解。我们证明，皮层的次级感觉区的电刺激显着减少了福尔马林诱导的c-Fos在SpVc中的表达的数量。神经元型一氧化氮合酶（n-NOS）和烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）在三叉神经感觉核的离散神经元群中表达。咬肌神经切断诱导三叉神经运动核和中脑核NADPH-d重新表达。n-NOS的表达与NADPH-d的表达相似，但延迟约一周。免疫组织化学分布的钙结合蛋白，钙结合蛋白，钙视网膜蛋白和小清蛋白和酶组织化学分布的NADPH-d已被检查。少

A member of DNA binding proteins, c-Fos was upregulated in neurons of the trigeminal sensory nucleus after electrical stimulation of the trigeminal ganglion. The expression was dependent on the intensify of the stimulation and the positive neurons were distributed in a nucleus specific manner. In the subnucleus caudalis (SpVc), most labeled neurons were in the superficial zone in its full extetent of the dorsomedial ventrolateral axis. In the oral subnucleus, c-Fos-positive neurons appeared only in the dorsomedial subnucleus. The c-Fos expression induced by a low intensity stimulation (0.1 mA), which activates A-beta fibers, and a high intensity stimulation (1.0 mA) which activates also C-fibers, was differently regulated by preadministration of GABAA and GABAB receptors agonist and antagonist. The segmental and supraspinal intensity-dependent GABA ergic regulation in the trigeminal sensory nucleus has been in plicated. Intrathecal administration of c-fos antisense oligodeoxynucleotide … More reduced adjuvant induced cFos expression and prolonged thermal hyperalgesia but not normal nociception. Thus c-Fos expression after nociception is involved in maintenance and/or development of hyperalgesia. Electrical stimulation of the somatosensory area of the cerebral cortex is applied in many patients suffering from intractable pain with good to excellent pain relief. We demonstrated that electrical stimulation of the secondary sensory area of the cortex significantly decreased the number of formalin-induced c-Fos expression in the SpVc. A neuronal nitric oxide synthase (n-NOS) and nicotinamide adenine dinucleotide phosohate-diaphorase (NADPH-d) is expressed in discrete population of neurons in the trigeminal sensory nucleus. Transection of masseteric nerve induced de novo expression of NADPH-d in the trigeminal motor nucleus and mesencephalic nucleus. The n-NOS expressed similarly with the NADPH-d expression, however delayed about one week. Immunohistochemical distribution of calcium binding proteins, calbindin, calretinin and parvalbumin and enzyme histochemical distribution of NADPH-d has been examined. Less