HISTOCHEMICAL MAPPING OF NADPH DIAPHORASE IN MONKEY EYES

猴眼 NADPH 心肌酶的组织化学图谱

• 批准号: 6116479
• 负责人: PAUL L KAUFMAN
• 金额: $ 5.34万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116479
• 关键词: animal tissue; enzymes; eye; vision

OBJECTIVE To localize in normal monkey eyes the enzyme involved in the synsthesis of nitric oxide RESULTS NADPH-diaphorase is widespread in monkey eyes indicating significant nitric oxide production in various ocular structures DISCUSSION Frozen sections of the eyes were mounted on slides, then fixed with 4% paraformaldehyde. NADPH diaphorase histochemistry was performed using nitroblue tetrazolium. Staining was found in the anterior and posterior ocular segment. In the anterior segment, reaction product was present in the corneal epithelium and endothelium, iris dilator and sphincter, lens capsule, ciliary muscle and non-pigmented epithelial cells and stroma of the ciliary processes. Trabecular meshwork staining was minimal if at all. In the posterior segment, staining was seen around choroidal blood vessels and choroidal nerves, and in the retinal pigmented epithelial, photoreceptor, bipolar, inner plexiform and ganglion cell layers FUTURE DIRECTIONS The role of nitric oxide in the normal physiology of aqueous humor formation and drainage and the pathophysiology of glaucoma will be studied. KEY WORDS nitric oxide, trabecular meshwork, ciliary muscle, ciliary processes FUNDING NEI EY02698, GRF PUBLICATIONS Chen Z, Gu Q, Kaufman PL, Cynader MS Histochemical mapping of NADPH-diaphorase in monkey and human eye. Curr Eye Res 17:370-379, 1998. [J]

目的：定位正常猴眼内的相关酶， 结果NADPH黄递酶广泛存在 在猴子的眼睛中， 各种眼部结构讨论眼睛的冷冻切片， 固定在载玻片上，然后用4%多聚甲醛固定。 NADPH 使用硝基蓝四唑进行心肌黄酶组织化学。 在眼前段和眼后段发现染色。 在 眼前节，反应产物存在于角膜中 上皮和内皮，虹膜扩张器和括约肌，透镜囊， 睫状肌和非色素上皮细胞和基质的 睫状突 小梁网染色极轻微，如果 所有. 在后段，染色可见脉络膜周围 血管和脉络膜神经，并在视网膜色素沉着 上皮细胞、感光细胞、双极细胞、内丛状细胞和神经节细胞 一氧化氮在正常人肝组织中的作用 生理学的房水形成和排水和 将研究青光眼的病理生理学。 关键词一氧化氮， 小梁网，睫状肌，睫状突 EY 02698，GRF出版物Chen Z，Gu Q，考夫曼PL，Cynader MS 猴和人眼中NADPH黄递酶的组织化学定位。 Curr Eye Res 17：370-379，1998。 [J]