The role of immunocompetent cells and cytokine network in the cystic and bone resorption.

应用免疫组织化学方法检测了3例成釉细胞瘤的颌骨囊壁(牙源性角化囊肿15例，根性囊肿5例)和结缔组织包膜中免疫活性细胞的分布。1.识别CD_4~+/CD_8~+T淋巴细胞的抗体UCHL-1在囊壁上皮下炎性病变中高表达。在非炎性囊壁中，UCHL-1阳性细胞倾向于定位于最外层的邻近骨质的区域。2.在纤维壁的血管周围区和炎性灶中可观察到识别多种单核巨噬细胞系的KP1抗体。在骨组织中，新生骨周围的单个核细胞和破骨细胞似乎表达该抗体。3.在邻近骨的纤维组织中观察到针对驻留巨噬细胞的HAM56抗体，尤其是血管周围的细胞。骨组织中的单个核细胞也表达HAM56抗体，但数量少于KP1阳性细胞，无破骨细胞表达该抗体。4.识别具有L1抗原的白细胞的MAC387在炎症灶和部分衬里上皮中强烈表达，而在纤维囊壁和骨组织中未见表达，推测免疫活性细胞的分布与囊变的生长有一定关系。

The distribution of immunocompetent cells in the wall of jaw cysts (15 odontogenic keratocysts and 5 radicular cysts) and the connective tissue capsule of 3 ameloblastomas were examined immunohistochemically.1.The antibody UCHL-1, which recognized CD4^+/CD8^+T lymphocytes, was intensely expressed in the subepithelial inflammatory lesion of the cyst wall. In the non-inflamed cyst wall, UCHL-1-positive cells tended to be localized in the outermost area adjacent to the bone.2.KP1 antibody which recognized a wide range of monocyte-macrophage lineage was observed in the perivascular area of fibrous wall as well as inflammatory foci. In the bone tissue, mononuclear cells around the newly formed bone and osteoclasts appeared to expressed this antibody.3.HAM56 antibody for resident macrophages was observed in the fibrous tissue adjacent to the bone, particularly the cells in the perivascular area. Mononuclear cells in the bone tissue also expressed HAM56 antibody, but they were fewer than KP1-positive cells and no osteoclasts expressed this antibody.4.MAC387 which recognized leukocytes having antigen L1 was intensely expressed in the inflammatory foci and a part of lining epithelium but no expression was discernible in the fibrous cyst wall and bone tissue.From the results to date it is presumed that there is a certain relationship between the distribution of immunocompetent cells and growth of the cystic lesion.