The role of immunocompetent cells and cytokine network in the cystic and bone resorption.

免疫活性细胞和细胞因子网络在囊性和骨吸收中的作用。

• 批准号: 06671890
• 负责人: YAMASAKI Akira
• 金额: $ 1.34万
• 依托单位: Ohu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671890/
• 关键词: Odontogenic keratocyst; Bone resorption; Immunocompetent cells; Macrophage; Lymphocyte; Immunocytochemistry

The distribution of immunocompetent cells in the wall of jaw cysts (15 odontogenic keratocysts and 5 radicular cysts) and the connective tissue capsule of 3 ameloblastomas were examined immunohistochemically.1.The antibody UCHL-1, which recognized CD4^+/CD8^+T lymphocytes, was intensely expressed in the subepithelial inflammatory lesion of the cyst wall. In the non-inflamed cyst wall, UCHL-1-positive cells tended to be localized in the outermost area adjacent to the bone.2.KP1 antibody which recognized a wide range of monocyte-macrophage lineage was observed in the perivascular area of fibrous wall as well as inflammatory foci. In the bone tissue, mononuclear cells around the newly formed bone and osteoclasts appeared to expressed this antibody.3.HAM56 antibody for resident macrophages was observed in the fibrous tissue adjacent to the bone, particularly the cells in the perivascular area. Mononuclear cells in the bone tissue also expressed HAM56 antibody, but they were fewer than KP1-positive cells and no osteoclasts expressed this antibody.4.MAC387 which recognized leukocytes having antigen L1 was intensely expressed in the inflammatory foci and a part of lining epithelium but no expression was discernible in the fibrous cyst wall and bone tissue.From the results to date it is presumed that there is a certain relationship between the distribution of immunocompetent cells and growth of the cystic lesion.

免疫组化检测颌骨囊肿（15个牙源性角化囊肿和5个根性囊肿）壁和3例成釉细胞瘤结缔组织囊中免疫活性细胞的分布。1.识别CD 4 ^+/CD 8 ^+T淋巴细胞的抗体UCHL-1在囊壁上皮下炎症性病变中强烈表达。在非炎症的囊肿壁中，UCHL-1阳性细胞倾向于定位于最外层的骨附近区域。2.在纤维壁的血管周围区域以及炎症灶中观察到识别广泛的单核-巨噬细胞谱系的KP 1抗体。在骨组织中，新生骨周围的单核细胞和破骨细胞似乎表达该抗体。3.在邻近骨的纤维组织中观察到针对驻留巨噬细胞的HAM 56抗体，特别是在血管周围区域的细胞中。骨组织中的单核细胞也表达HAM 56抗体，但他们比KP 1少4.MAC387识别具有抗原L1的白细胞，在炎症灶和部分衬里上皮中强烈表达，但在纤维囊肿壁和骨组织中没有可辨别的表达。免疫活性细胞的分布与囊性病变生长的关系。