Involvement of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases in Periodontal Tissue Destruction

基质金属蛋白酶和金属蛋白酶组织抑制剂参与牙周组织破坏

• 批准号: 11671809
• 负责人: YAMASAKI Akira
• 金额: $ 2.24万
• 依托单位: Ohu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671809/
• 关键词: MMP-1; TIMP-1; radicular cyst; odontogenic keratocyst; immunohistochemistry; 歯周組織; メタロプロテアーゼ; in situ hybridization; 嚢胞

The immunohistochemical study on the expression of matrix metalloproeinase (MMP)-1, MMP-8, and tissue inhibitor of matrix proteinase (TIMP)-1 in the cyst wall was designed to understand the mechanism of the cyst growth. Total 68 cases of radicular cyst and 13 cases of odontogenic keratocyst were examined.Fibroblasts, capillary endothelial cells, macrophages, and cells of the lining epithelium in both radicular cysts and odontogenic keratocysts commonly expressed MMP-1 and TIMP-1 but not MMP-8. In radicular cysts, the positive cell rate for MMP-1 to total cells comprising the connective tissue wall, except for lymphocytes and plasma cells, was significantly higher than that for TIMP-1, the difference being more pronounced in the resting type of radicular cyst (71,2% for MMP-1 and 15.9% for TIMP-1) than inflamed type (64.8% for MMP-1 and 43.2% for TIMP-1). There was no difference in the lining epithelium of radicular cyst, in which most of the cells appeared to be immunoreactive for both MMP-1 and TIMP-1. In odontogenic keratocyst, in contrast, higher positive cell rate for MMP-1 compared to that for TIMP-1 was found in the lining epithelium and there was no significant difference in the connective tissue wall.From the present results, it is suggested that the disruption of the balance between MMP-1 and TIMP-1 activities may be responsible for tissue destruction associated with the cyst growth. The change takes place in the connective tissue wall in radicular cyst and in the lining epithelium in odontogenic keratocyst, respectively, suggesting that there is a different growth mechanism between the both.

应用免疫组织化学方法对囊壁基质金属蛋白酶-1、8和基质金属蛋白酶组织抑制因子-1的表达进行研究，以了解囊壁生长的机制。对68例根尖囊肿和13例牙源性角化囊肿中的成纤维细胞、毛细血管内皮细胞、巨噬细胞和衬里上皮细胞共同表达MMP1和TIMP-1，而不表达MMP8。在根尖囊肿中，除淋巴细胞和浆细胞外，其阳性细胞数占结缔组织壁细胞总数的比例明显高于TIMP-1，静止型(71.2%和15.9%)明显高于炎症型(64.8%和43.2%)。神经根囊肿衬里上皮细胞与基质金属蛋白酶-1和基质金属蛋白酶-1免疫反应阳性细胞的分布无明显差异。在牙源性角化囊肿中，基质金属蛋白酶-1的阳性细胞率高于基质金属蛋白酶-1，在结缔组织壁中的阳性细胞率无显著差异，提示基质金属蛋白酶-1/基质金属蛋白酶-1活性平衡的破坏可能是与囊肿生长相关的组织破坏的原因。根尖囊中结缔组织壁和牙源性角化囊中衬里上皮分别发生了这种变化，提示两者存在不同的生长机制。