Elucidation of the caries-inducing mechanism by S.mutans and development of a diagnostic method to estimate caries-risk.

阐明变形链球菌诱发龋齿的机制并开发估计龋齿风险的诊断方法。

基本信息

项目摘要

Recentry, we have constructed S.milleri transformants KSB8, KSC43 and NH5 which individually express S.mutans gtfB,gtfC and gtfD coding GTF-I,-SI and -S enzymes, and established hybridoma which produce monoclonal antibodies mono-specific to the three GTFs. In this study, in vitro and in vivo cariogenicities of the three transformants were compared to elucicate the caries-inducing mechanism by S.mutans. Furthermore, using the mono-specific MAbs, a high-sensitive Gross-dot method for determination of the three GTFs was developed. The results obtained were as follows :1. More stable gtfC-expressing transformant KSC43-RT was reisolated from an oral cavity of rat infected with KSC43.2. In vitro cariogenicity of the transformants was examined by a colonization test. The results showed that only KSC43-RT cells could makedly colonized on glass surfaces.3. In vivo cariogenicity of the transformants was tested by a rat model system. The results showed that the KSC43-RT cells could induced the buccal and sulcal caries, and the KSB8 cells could induced the proximal caries, but the NH5 cells did not.4. High-sensitive Gross-dot assay method was established and applied in order to evaluate the cariogenicity of S.mutans strains.5. Cell-associated GTFs from various clinical isolates of S.mutans were analyzed by the method. The results showed that the levels of three GTFs and those ratio were markedly different among the isolates.These results suggest that the gtfC product was especially important for caries development caused by S.mutans, and that the high-sensitive Gross-dot method may be useful to estimate the exact cariogenicity of individual strain of S.mutans.
最近,我们构建了分别表达编码GTF-I、-SI和-S酶的变形链球菌gtfB、gtfC和gtfD的米勒链球菌转化子KSB 8、KSC 43和NH 5,并建立了产生对这三种GTF单特异性的单克隆抗体的杂交瘤。在这项研究中,在体外和体内致龋性的三个转化子进行了比较,阐明致龋的变异链球菌的机制。此外,使用单特异性单克隆抗体,一个高灵敏度的Gross-dot方法测定的三个GTF。主要研究结果如下:1.从感染KSC 43.2的大鼠的口腔中重新分离更稳定的表达gtfC的pETKSC 43-RT。通过定殖试验检查转化体的体外致龋性。结果表明,只有KSC 43-RT细胞能在玻璃表面形成定植.通过大鼠模型系统测试转化体的体内致龋性。结果表明,KSC 43-RT细胞能诱导颊面龋和沟龋,KSB 8细胞能诱导邻面龋,而NH 5细胞不能诱导邻面龋.建立并应用高灵敏度的Gross-dot法评价变形链球菌的致龋性.通过该方法分析了来自各种变形链球菌临床分离株的细胞相关GTF。结果表明,不同菌株的GTFs含量及其比值存在明显差异,提示gtfC产物在变形链球菌致龋过程中起重要作用,高灵敏度的Gross-dot法可用于准确评价变形链球菌的致龋性。

项目成果

期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
福島和雄: "Streptococcus mutans群Glucosyltransferaseの分子生物学-虫歯発症機序の解析-" 応用糖質科学. 第42巻. 157-167 (1994)
Kazuo Fukushima:“变形链球菌葡萄糖基转移酶的分子生物学 - 龋齿发生机制的分析”Applied Glycoscience Vol. 42. 157-167 (1994)
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Fukushima, K., Namiki Y.and Ikemi T.: "Semi-quantitative cross-dot assay for three glucosyltransterases of Streptococcus mutans." Dentistry in japan. 31. 27-29 (1994)
Fukushima, K.、Namiki Y. 和 Ikemi T.:“变形链球菌三种葡萄糖基转移酶的半定量交叉点测定。”
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Fukushima K., Namiki Y. and lkemi T.: "Semi-quantitative Cross-dot Assay for Three Glucosyltransferases of Streptococcus mutans." Dentistry in Japan. Vol.31. 27-29 (1994)
Fukushima K.、Namiki Y. 和 lkemi T.:“变形链球菌三种葡萄糖基转移酶的半定量交叉点测定。”
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Fukushima, K.: "Molecular biology of mutans streptococcal glucosyltransferase--Analysis of caries-inducing mechanism--" Oyo Toshitu Kagaku. 42. 157-167 (1994)
Fukushima, K.:“变形链球菌葡萄糖基转移酶的分子生物学——诱发龋齿的机制分析——”Oyo Toshitu Kagaku。
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K.Fukushima,Y.Namiki and T.Ikemi: "Semi-quantitative Cross-dot Assay for Glucosyltransferases of Streptococcus mutans" Dentistry in Japan. 31(in press). (1994)
K.Fukushima、Y.Namiki 和 T.Ikemi:“变形链球菌葡萄糖基转移酶的半定量交叉点测定”日本牙科。
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FUKUSHIMA Kazuo其他文献

FUKUSHIMA Kazuo的其他文献

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{{ truncateString('FUKUSHIMA Kazuo', 18)}}的其他基金

Developments of rapid immuno-chromato kits for caries-risk estimation using anti-GTF monoclonal antibodies
使用抗 GTF 单克隆抗体开发用于龋齿风险评估的快速免疫色谱试剂盒
  • 批准号:
    15591950
  • 财政年份:
    2003
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Construction and Isolation of streptococci useful for caries-prevention by replacement therapy
用于通过替代疗法预防龋齿的链球菌的构建和分离
  • 批准号:
    12671786
  • 财政年份:
    2000
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
RESEARCH ON BIOMARKERS RELEASED BY PYROLYSIS OF THE INSOLUBLE ORGANIC MATTER IN GEOCHEMICAL SAMPLES.
地球化学样品中不溶性有机物热解释放的生物标志物的研究。
  • 批准号:
    10440159
  • 财政年份:
    1998
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
INVESTIGATION ON THE BIOLOGICAL AND CHEMICAL MARKERS OF THE ACIDIFICATION OF LAND WATERS
陆地水域酸化的生物和化学标志物研究
  • 批准号:
    05453196
  • 财政年份:
    1993
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Gene Cloning of Oral Streptococcal Glucanases
口腔链球菌葡聚糖酶的基因克隆
  • 批准号:
    60570859
  • 财政年份:
    1985
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Beta3-葡萄糖基转移酶在非规范质量控制途径中的作用
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