Construction and Isolation of streptococci useful for caries-prevention by replacement therapy

用于通过替代疗法预防龋齿的链球菌的构建和分离

• 批准号: 12671786
• 负责人: FUKUSHIMA Kazuo
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671786/
• 关键词: Caries Prevention; Replacement therapy; Streptococcus mutans; Streptococcus sobrinus; Biofilm; S. mutans; S. sobrinus

Mutans streptococci, Streptococcus mutans and Streptococcus sobrinus, have been considered the principal etiological agents of dental caries. The degree of colonization of these organisms correlates with the prevalence of dental carries in, human and experimental animals. An important virulence factor of mutans streptococci promoting caries development is the abilities to colonize firmly to the tooth surface in the presence of sucrose and produce the cariogenic biofilm in situ.The purpose of this research project was to construct or to isolate anti-cariogenic I streptococci useful for caries prevention by replacement therapy. The results have shown that a S. mutans MKM33 isolated from a caries-free student was colonizable and non-cariogenic in rats, that two S. sobrinus like isolates (HG1006 and HG988) from Dutch childs were identified as S. cricetus and both isolates also were colonizable and non-cariogenic in rats, and that a S. gordonii transformant expressing a cyclodextran-synthesizing enzyme gene was colonizable in mice. These findings suggest that the clinical isolates. MKM33, HG1006 and HG988 might be useful for caries prevention by replacement therapy. In addition, some useful microbiological methods for isolation of mutans streptococci were established, and many S. mutans were isolated from students with zero or low of caries experience, but S. sobrinus did not.

变形链球菌和远缘链球菌被认为是龋病的主要病原体。这些微生物的定殖程度与人类和实验动物中牙齿携带的患病率相关。变形链球菌促进龋病发展的一个重要毒力因子是在蔗糖存在下牢固地定殖到牙齿表面并原位产生致龋生物膜的能力，本研究的目的是构建或分离抗致龋I型链球菌，用于替代疗法预防龋病。结果表明，S.从一名无龋学生中分离的变形链球菌MKM 33在大鼠中是可定殖的且非致龋性的，两株变形链球菌MKM 33在大鼠中是可定殖的且非致龋性的。从荷兰查尔兹儿童分离的类远缘链球菌（HG 1006和HG 988）被鉴定为S. cricetus和这两个分离株在大鼠中也是可定殖的和非致龋的，并且S.表达环葡聚糖合成酶基因的戈登氏菌在小鼠中是可定殖的。这些发现表明临床分离株。MKM 33、HG 1006和HG 988可作为替代治疗的防龋剂。此外，还建立了一些分离变形链球菌的微生物学方法。无龋史或低龋史的学生中分离出变形链球菌，而S.索布里努斯没有。

项目成果

Okada, T., Tomita, Y., Ikemi, T., Fukushima, K. et al.: "In vitro cariogenicity test of Streptococcus mutans isolated from caries-susceptible and caries-free students"Jap. J. Conserv. Dent.. Vol.43(5). 1113-1120 (2000)

Okada, T.、Tomita, Y.、Ikemi, T.、Fukushima, K. 等：“从易患龋齿和无龋齿的学生中分离出的变形链球菌的体外致龋性试验”

岡田珠美, 富田行秀, 田中秀雄, 藤崎宣子, 河野善治, 池見宅司, 竹内武男, 福島和雄: "高う蝕者と無う蝕者から分離したStreptococcus mutansのin vitroう蝕原性試験"日本歯科保存学雑誌. 43巻5号. 1113-1120 (2000)

Tamami Okada、Yukihide Tomita、Hideo Tanaka、Nobuko Fujisaki、Yoshiharu Kono、Takuji Ikemi、Takeo Takeuchi、Kazuo Fukushima：“从易患龋齿和非龋齿患者中分离出的变形链球菌的体外致龋性测试”《日本保守牙科杂志》卷。 43，第5号。1113-1120（2000）

Neta, T., Inokuchi, R., Shinozaki, N., Kouno, Y., Ikemi, T., Fukushima, K: "Investigation of Microbiological Methods of Estimating Individual Caries Risk : Evaluation of Sampling Methods and Materials"Int. J. Oral Biol. Med.. Vol.1No.1(In press). (2002)

Neta, T.、Inokuchi, R.、Shinozaki, N.、Kouno, Y.、Ikemi, T.、Fukushima, K：“估计个体龋齿风险的微生物方法的调查：采样方法和材料的评估”Int。

Neta, T., Inokuchi, R., Kuwahara-Shinozaki, N., Kouno, Y., Ikemi, T., Fukushima, K.: "Investigation of Microbiological Methods of Estimating Individual Caries Risk : Evaluation of Sampling Methods and Materials"Int.J.Oral Biol.Med.. Vol.1 No.1(in press).

Neta, T.、Inokuchi, R.、Kuwahara-Shinozaki, N.、Kouno, Y.、Ikemi, T.、Fukushima, K.：“估计个体龋齿风险的微生物方法的调查：采样方法和材料的评估”

Neta, T., Inokuchi, R., Kuwahara-Shinozaki, N., Fukushima, K. et al.: "Investigation of microbiological methods of estimating individual caries risk : Evaluation of sampling methods and materials"Int. J. Oral Biol. Med.. Vol.1(1) (in press).

Neta, T.、Inokuchi, R.、Kuwahara-Shinozaki, N.、Fukushima, K. 等人：“估计个体龋齿风险的微生物方法的调查：采样方法和材料的评估”Int。