Developments of rapid immuno-chromato kits for caries-risk estimation using anti-GTF monoclonal antibodies

使用抗 GTF 单克隆抗体开发用于龋齿风险评估的快速免疫色谱试剂盒

• 批准号: 15591950
• 负责人: FUKUSHIMA Kazuo
• 金额: $ 2.3万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591950/
• 关键词: Streptococcus mutans; Streptococcus sobrinus; Caries risk; ELISA; GTF-B; GTF-I; Monoclonal antibody; Rapid diagnosis; S.mutans; S.sobrinus; モノクローナル

Streptococcus mutans and Streptococcus sobrinus secrete glucosyltransferase(GTF)-B and GTF-I, which are considered to be the important factors of cariogenic biofilm formation. In order to deve-lope the rapid immuno-chromato kits for caries-risk estimation using anti-GTF monoclonal antibodies, we done the improvment of the GTF-B detection system consists of sandwich enzyme-linked immunosorbent assay(ELISA) using a mouse monoclonal and a rabbit polyclonal antibodies against GTF-B. The recombinant (r) GTF-B was highly purified from culture fluids of KSB8 transformant and used forcariblation of the developed ELISA system. This assay system could quantify with accuracy small amounts (1-100 ng)of the purified rGTF-B. In addition, we analyzed GTF-B productivity of S. mutans by this ELISA systemand indicate d that this system is a good way as examination of the GTF-B level in culture samples, and suggested that this may be useful tool for quantification of the amount of GTF-B in clinical plaque samples. Furthermore、we developed the GTF-I detection system consists of sandwich enzyme-linked immunosorbent assay(ELISA) using a mouse monoclonal and a rabbit polyclonal antibodies against GTF-I.

链球菌突变体和Sobrinus秘密葡萄糖基转移酶（GTF）-b和GTF-I，它们被认为是肉源性生物膜形成的重要因素。为了开发使用抗GTF单克隆抗体的快速免疫 - 纤维充电试剂盒，用于龋齿风险估算，我们使用小鼠单核和兔polyclonal antbibodies对GTF-B检测系统进行了改善GTF-B检测系统的改进。重组（R）GTF-B从KSB8转化剂的培养液中高度纯化，并使用了发达的ELISA系统的强迫。该测定系统可以准确地量化纯化的RGTF-B的少量（1-100 ng）。此外，我们通过该ELISA系统分析了链球菌的GTF-B生产率，并表明该系统是对培养样品中GTF-B水平的检查的好方法，并建议这可能是量化临床斑块样品中GTF-B量的有用工具。此外，我们开发了GTF-I检测系统由使用小鼠单克隆和针对GTF-I的兔多克隆抗体组成的三明治酶联免疫吸附测定法（ELISA）。

项目成果

う蝕性リスクの判定方法及び判定薬

确定龋齿风险的方法和药物

• 发表时间: 2004

Estimation of Streptococcus mutansin brushing plaques from students and childs by a Lapid immunoassay kit.

通过 Lapid 免疫测定试剂盒估算学生和儿童刷牙斑块中的变形链球菌。

• 期刊: J.Pediat.Dent. 43(1)
• 作者: K.Mizuno;et al.
• 通讯作者: et al.

大学生および児童からのブラッシング歯垢を検体とするS.mutans迅速測定キットの有用性評価

以大学生和儿童的刷牙牙菌斑为标本，评价变形链球菌快速检测试剂盒的实用性

• 发表时间: 2005
• 期刊: 小児歯科学雑誌 43(1)
• 作者: N.Shinozaki-Kuwahara;et al.;水野恭子他
• 通讯作者: 水野恭子他

Expression of the gtfI gene from Streptococcus sobrinus in Streptococcus anginosus using integration-mediated transformation system.

使用整合介导的转化系统在咽峡炎链球菌中表达来自 Sobrinus 链球菌的 gtfI 基因。

• 发表时间: 2005
• 期刊: Biochemica et Biophysica Acta 1722
• 作者: N.Shinozaki-Kuwahara;et al.
• 通讯作者: et al.