Developments of rapid immuno-chromato kits for caries-risk estimation using anti-GTF monoclonal antibodies

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Streptococcus mutans and Streptococcus sobrinus secrete glucosyltransferase(GTF)-B and GTF-I, which are considered to be the important factors of cariogenic biofilm formation. In order to deve-lope the rapid immuno-chromato kits for caries-risk estimation using anti-GTF monoclonal antibodies, we done the improvment of the GTF-B detection system consists of sandwich enzyme-linked immunosorbent assay(ELISA) using a mouse monoclonal and a rabbit polyclonal antibodies against GTF-B. The recombinant (r) GTF-B was highly purified from culture fluids of KSB8 transformant and used forcariblation of the developed ELISA system. This assay system could quantify with accuracy small amounts (1-100 ng)of the purified rGTF-B. In addition, we analyzed GTF-B productivity of S. mutans by this ELISA systemand indicate d that this system is a good way as examination of the GTF-B level in culture samples, and suggested that this may be useful tool for quantification of the amount of GTF-B in clinical plaque samples. Furthermore、we developed the GTF-I detection system consists of sandwich enzyme-linked immunosorbent assay(ELISA) using a mouse monoclonal and a rabbit polyclonal antibodies against GTF-I.