動物組織のリン脂質分子種組成の独立性と外的因子による制御

动物组织中磷脂分子种类组成的独立性及受外界因素的控制

• 批准号: 06672175
• 负责人: KAWASHIMA Yoichi
• 金额: $ 1.15万
• 依托单位: Josai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672175/
• 关键词: Phospholipid; Molecular species; Clofibrate; Tissue independence

Compoition of molecular species of phospholipid regulates physical properties of biological membranes and affects signal transduction and formation of eicosanoids in cells. Accordingly, one could regulate physiology ofa particular organ through the control of compositions of molecular species of phospholipids if one has a way to manipulate the composition. In this context, we studied the mechanism for the control of composition of molecular species of phospholipids in organs and obtained the following results.(1)Clofibric acid increased hepatic concentrations of phosphatidylcholine (PC) by enhancing bioynthesis de novo and of phosphatidylethanolamine (PE) by stimulating a subroute from PC to PE via phosphatidylserine. The metabolic changes induced by clofibric acid in hepatic phospholipids caused marked alterations of composition of molecular species of PC and PE,resulting from concerted effects of changes in composition of molecular speciss of diacylglycerol supplied, stimulation of reacylation of PC and PE,and suppression of N-methylation of PE.(2) kidney contained massive dipalmitoy-PC that liver and serum hardly contained, which is an originality of kidney concerning composition of molecular species of PC.(3) Effects of clofibric acid, feeding fat free diet and induction of diabetes on the compositions of molecular species of PC in brain, pancreas and kidney were investigated. No alteration was observed in brain, indicationg that composition of molecular species of phospholipid of brain in under strict control and is independent of changes occurred in serum phospholipid. The composition of molecular species of renal PC was strongly dependent on that of serum PC and the composition of molecular species of pancratic PC depended partially on that of serum phospholipid. Consequently, these results are indicative of the possibility that composition of molecular species of phospholipids can be manipulated by drug (such as clofibric acid) and dietary fatty acids.

磷脂分子种类的组成调节生物膜的物理性质，影响细胞内的信号转导和类二十烷的形成。因此，如果有办法操纵磷脂分子种类的组成，就可以通过控制磷脂分子种类的组成来调节特定器官的生理。在此背景下，我们研究了控制器官磷脂分子种类组成的机制，得到了以下结果。(1)纤维素酸通过刺激磷脂酰丝氨酸从磷脂酰胆碱到磷脂酰乙醇胺（PE）的亚路线，促进磷脂酰胆碱（PC）的生物新合成和磷脂酰乙醇胺（PE）的合成，从而提高肝脏磷脂酰胆碱（PC）浓度。纤维酸诱导的肝脏磷脂代谢变化引起了PC和PE分子种类组成的显著改变，这是由于供体二酰基甘油分子种类组成的变化、PC和PE的再酰化的刺激以及PE n -甲基化的抑制共同作用所致。(2)肾脏中含有大量的双掌肌-PC，这是肝脏和血清中几乎不含的，这是肾脏在PC分子种类组成方面的一个独创性。(3)研究了纤维酸、饲喂无脂饲料和糖尿病诱导对大鼠脑、胰腺和肾脏PC分子组分的影响。脑内未见改变，说明脑内磷脂分子种类的组成受到严格控制，与血清磷脂的变化无关。肾脏PC分子种类的组成强烈依赖于血清PC，胰腺PC分子种类的组成部分依赖于血清磷脂。因此，这些结果表明磷脂分子种类的组成可以被药物（如纤维酸）和膳食脂肪酸操纵的可能性。