权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Study on the variety of the effects of perfluorinated fatty acids on biological systems and the biological mechanisms for identification of each molecules.

研究全氟脂肪酸对生物系统的多种影响以及各分子的生物学机制。

基本信息

批准号：
09672247
负责人：
KAWASHIMA Yoichi
金额：
$ 2.11万
依托单位：
Josai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

The effects of perfluorinated fatty acids (PFCAs) having 7-10 carbon atoms were compared in rats. PFCAs having 8-10 carbon atoms lowered serum levels of lipids. PFCA having 9 and 10 carbon atoms caused accumulation of lipid droplets in the liver and renal proximal tubular cells in rats.In the liver of rats treated with PFCA having 10 carbon atoms, fatty acid synthesis and β-oxidation was accelerated whereas secretion of VLDL was suppressed. Therefore, the accumulation of triglyceride (TG) is thought to be, at least partly, due to suppression of VLDL secretion and acceleration of fatty acid synthesis.When the potency of the induction of peroxisomal β-oxidation and accumulation of TG in rat liver was compared, PFCA having longer carbon chain length was more potent. Sex-related difference was observed in the effects of PFCAs having 8 and 9 carbon atoms. There are highly significant correlations between hepatic concentrations of PFCA and the activity of peroxisomal β-oxidation or hepatic TG content regardless of the carbon chain length of PFCA, suggesting that difference in the accumulation in liver is responsible for the difference in the potency of the induction of peroxisomal β-oxidation and TG accumulation in rats. Essentially the same result was obtained from mice.PFCA having shorter carbon chain length was more quickly excreted into urine. Female rats excreted PFCA faster than male rats. By contrast, excretion rates of PFCAs in feces were slow compared to those of urine and there was no significant difference between PFCAs having different carbon chain length and between sexes. Urinary excretion rate was regulated by testosterone in male rats. The results indicate that the difference in the accumulation between PFCAs having 7-9 carbon atoms is due to difference in the rate of urinary excretion, which is regulated by testosterone.

在大鼠中比较了具有7-10个碳原子的全氟脂肪酸（PFCAs）的影响。具有8-10个碳原子的PFCA降低血清脂质水平。含9和10个碳原子的PFCA可引起大鼠肝和肾近端小管细胞内脂滴的积聚，含10个碳原子的PFCA可促进大鼠肝内脂肪酸的合成和β-氧化，抑制VLDL的分泌。因此，认为甘油三酯（TG）的蓄积至少部分是由于抑制VLDL分泌和加速脂肪酸合成所致。当比较大鼠肝脏中过氧化物酶体β-氧化和TG蓄积的诱导效力时，具有较长碳链长度的PFCA更有效。碳原子数为8和9的PFCAs的作用存在性别差异。无论PFCA的碳链长度如何，肝脏中PFCA浓度与过氧化物酶体β-氧化活性或肝脏TG含量之间均存在高度显著相关性，表明肝脏中蓄积的差异是导致大鼠体内过氧化物酶体β-氧化和TG蓄积诱导效力差异的原因。在小鼠中也得到了基本相同的结果，碳链长度较短的PFCA更快地排泄到尿液中。雌性大鼠排泄PFCA的速度比雄性大鼠快。相比之下，粪便中PFCAs的排泄率比尿液中的排泄率慢，不同碳链长度的PFCAs之间和性别之间没有显着差异。雄性大鼠尿排泄率受睾酮调节。结果表明，具有7-9个碳原子的PFCA之间的积累差异是由于尿排泄速率的差异，这是由睾酮调节的。