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Descending projections of the hypothalamic paraventricular nucleus to the parasympathetic preganglionic neurons

下丘脑室旁核到副交感神经节前神经元的下降投射

基本信息

批准号：
04670034
负责人：
HOSOYA Yasuhiko
金额：
$ 1.22万
依托单位：
University of Tsukuba, College of Medical Technology and Nursing Grant-in Aid for Scientific Research(C)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

The present study was undertaken to elucidate whether the descending fibers of the hypothalamic paraventricular nucleus have a direct connection with the parasympathetic pregaglionic neurons. At the first step of the experiment, we developed an operating technique to reach and expose the major pelvic gaglion of the male rat. After the major pelvic ganglion was exposed on the wall of the prostate gland, the retrograde labelling tracer, cholera toxin subunit B(CTB), was injected into the ganglion. This technique resulted in a retrograde labeling of many sympathetic and parasympathetic preganglionic neurons in the lumbar and sacral cords. In the sacral level, the primary sensory fibers that were labeled anterogradly were observed, in addition to the parasympathetic preganglionic neuros. At the lumbar cord level, many labeled sympathetic preganglionic neurons were obserbed in the midregion, just dorsal to the central canal. They formed a single cell column as the dorsal commissural nuclues. The nucleus appeared to be very similar with the Terni's nucleus of the chick in respects of cell location, forming single cell column. A signle cell column situated in the midline seens to be suitable to innervate unpaired pelvic organs. This results will be published in "The Experimental Brain Research" this year. Although the morphology of the dorsal commissural nucleus was shown, we were not able to clarify the neuronal connections between the hypothalamic paraventricular nuclues and the sacral parasympathetic preganglionic neurons.

本研究旨在阐明下丘脑室旁核的下行纤维是否与副交感神经节前神经元有直接联系。在实验的第一步，我们开发了一种手术技术，以达到并暴露雄性大鼠的主要盆腔梗阻。将盆腔大神经节暴露于前列腺壁上后，向神经节内注射霍乱毒素亚单位B（CTB）逆行标记示踪剂。这项技术导致许多交感和副交感神经节前神经元在腰椎和骶索逆行标记。在骶骨水平，除了副交感神经节前神经外，还观察到顺行标记的初级感觉纤维。在腰椎水平，许多标记的交感神经节前神经元在正中区，正中管背侧被观察到。它们形成一个单细胞柱作为背交核。细胞核在细胞位置上与鸡的Terni细胞核非常相似，形成单细胞柱。位于中线的单细胞柱似乎适合支配未配对的盆腔器官。这一研究结果将发表在今年的《实验大脑研究》上。虽然显示了背交核的形态，但我们无法阐明下丘脑室旁核与骶部副交感神经节前神经元之间的神经元连接。