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Hypothalamic Projections to the Raphe Nuclei of the Medulla oblongata.

下丘脑对延髓中缝核的投射。

基本信息

批准号：
60570022
负责人：
HOSOYA Yasuhiko
金额：
$ 1.09万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570022/
关键词：
Hypothalamus Dorsal Hypothalamic Area Descending Projection Autoradiography HRP オートラジオグラフィー

项目摘要

The present study was undertaken using the anterograde and retograde labeling techniques to clarify the hypothalamic descending projections tothe medullary raphe nuclei in the rat.1. Anterograde labeling technique. A small amount of [ <^3H> ] leucine was injected unilaterally into various parts of the hypothalamus. Abundant and characteristic terminal labelings were observed bialterally in the nucleus raphe pallidus, the vental surface of the pyramidal tract and the nucleus interfascicularis hypoglossi, after injections into the dorsal posterior hypothalamic area caudal to the paraventricular hypothalamic nucleus. Conspicuous, but less numerous labelings were observed in the nucleus raphe obscurus and the ipsilateral raphe magnus.2. Retrograde labeling techniques. Following an injection of WGA-HRP in the nucleus raphe pallidus many labelled smalll neurons (6-13X9-22 <micro> m) with an oval shape were found in the ventromedial part of the dorsal hypothalamic area. At the level through the dorsomedial hypothalamic nucleus, they formed a distinct and compact cell cluster. Labelled neurons, which were large in size (9-22X11-36 <micro> m) with oval and triangular shapes, were found mainly in the dorsolateal part of the dorsal hypothalamic area after injections of HRP in the spinal cord. In a double-labeling experiment, Fast Blue or True Blue, and Nuclear Yellow were injected in the nucleus raphe pallidus and the spinal cord, respectively. A large number of blue-fluorescent neurons were located mostly in the ventromedial part of the dorsal hypothalamic area, while yellow-fluorescent ones were found in the dorsolateal part of the dorsal hypothalamic area.These results show that the dorsal hypothalamic area projects directly to the nucleus raphe pallidus, and that the neurons which project to the spinal cord differ from those which project to the nucleus raphe pallidus.

本研究应用顺行和逆行标记技术，阐明了大鼠下丘脑下行投射至延髓中缝核的投射.顺行标记技术。将少量[^3H> ]亮氨酸单侧注射到下丘脑的不同部位。丰富的和特征性的终端标记观察bialterally中缝苍白核，腹侧表面的锥体束和神经束间核舌下，注射到背侧后下丘脑区尾侧的下丘脑室旁核。在中缝隐核和同侧中缝大核中观察到明显但数量较少的标记。逆行标记技术。将WGA-HRP注入中缝苍白核后，在下丘脑背侧区腹内侧部发现许多椭圆形的标记小神经元（6- 13 × 9 -22 <micro>μ m）。在穿过下丘脑背内侧核的水平上，它们形成了一个清晰而紧凑的细胞簇。HRP<micro>注入脊髓后，标记神经元主要分布于下丘脑背侧区的背外侧部，其大小为9- 22 × 11 -36 μ m，呈椭圆形和三角形。在双标记实验中，分别在中缝苍白核和脊髓中注射固蓝或真蓝和核黄。在下丘脑背侧区的腹内侧部有大量的蓝色荧光神经元，而在下丘脑背侧区的背外侧部有大量的黄色荧光神经元，表明下丘脑背侧区直接投射到中缝苍白核，而且投射到脊髓的神经元与投射到中缝苍白核的神经元不同。