Effects of high-energy phosphates on morphological and functional development in rat muscles

高能磷酸盐对大鼠肌肉形态和功能发育的影响

• 批准号: 06807007
• 负责人: OHIRA Yoshinobu
• 金额: $ 1.09万
• 依托单位: National Institute of Fitness and Sports in Kanoya
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807007/
• 关键词: Creatine; High-energy phosphates; Metabolic properties; Contractile properties; ラット; 骨格筋; β-guanidinopropionic acid

Effects of chronic depletion or loading of high-energy phosphate contents in skeletal muscles by feeding either beta-guanidinopropionic acid (beta-GPA) or creatine were studied in Wistar rats. The contractile properties in both soleus and extensor digitorum longus (EDL) were shifted toward slower type by feeding beta-GPA.The fatigue resistance in these muscles, especially EDL,was also improved. Transition of myosin isoforms in fast-twitch plantaris muscle was induced by administration of beta-GPA.Greater glucose uptake and the maximum binding capacity of beta-adrenoceptor, due to an increased receptor number, were further observed in the musles of beta-GPA group. Administration of creatine induced, generally, opposite phenomena. It is suggested that chronic depletion of high-energy phosphates stimulates the mitochondrial energy metabolism and shifts the muscle towerd slower type. It is also indicated that maintenance of high-energy phosphates at higher levels may not be essential for the greater fatigue resistance, if the capacity of adenosine triphosphate synthesis is improved.

在Wistar大鼠中研究了通过喂食β-胍基丙酸（β-GPA）或肌酸来慢性消耗或负荷骨骼肌中高能磷酸盐含量的影响。β-GPA可使比目鱼肌和趾长伸肌（EDL）的收缩特性向慢收缩型转变，并能提高比目鱼肌和趾长伸肌的抗疲劳能力，尤其是趾长伸肌。注射β-GPA后，快速收缩的跖肌中肌球蛋白亚型发生转变，β-GPA组的葡萄糖摄取量和β-肾上腺素受体的最大结合量均明显增加，这是由于β-GPA组的受体数量增加所致。肌酸的管理引起，一般来说，相反的现象。提示高能磷酸盐的慢性消耗刺激了线粒体能量代谢，使肌肉向塔慢型转变。它还表明，维持高能量磷酸盐在较高的水平可能不是必不可少的更大的抗疲劳性，如果腺苷三磷酸合成的能力得到改善。

项目成果

Tanaka, M., F.Kariya, W.Yasui, K.Kaihatsu, T.Tanaka, amd Y.Ohira.: "Effects of creatine contents on behavior and brain metabolism in rats." 71st Ann.Meet.Jpn.Soc.Physiol.Mar.24,1994.

Tanaka, M.、F.Kariya、W.Yasui、K.Kaihatsu、T.Tanaka、amd Y.Ohira.：“肌酸含量对大鼠行为和脑代谢的影响。”

Wakatsuki,T.,Y.Ohira,et al.: "Responses of contractile properties in rat soleus to high-energy phosphates and/or unloading" Jpn.J.Physiol.44. 193-204 (1994)

Wakatsuki,T.,Y.Ohira,et al.：“大鼠比目鱼肌收缩特性对高能磷酸盐和/或卸载的反应”Jpn.J.Physiol.44。

Wakatsuki, T., Y.Ohira, W.Yasui, K.Nakamura, T.Asakura, H.Ohno, and M.Yamamoto.: "Responses of contractile properties in rat soleus to high-energy phosphates and/or unloading." Jpn.J.Physiol.44. 193-204 (1994)

Wakatsuki, T.、Y.Ohira、W.Yasui、K.Nakamura、T.Asakura、H.Ohno 和 M.Yamamoto.：“大鼠比目鱼肌收缩特性对高能磷酸盐和/或卸载的反应。”

Tanaka, M., K.Mizuta, Y.Ohira, and Y.Honda.: "Effects of exposure to hypobaric pressure on work capacity and enzyme activities in skeletal muscles of rats" 72nd Ann.Meet.Jpn.Soc.Physiol., Mar.31,1995.

Tanaka, M.、K.Mizuta、Y.Ohira 和 Y.Honda.：“暴露于低压对大鼠骨骼肌工作能力和酶活性的影响”第 72 届 Ann.Meet.Jpn.Soc.Physiol.，

Ren,J.-M.,Y.Ohira,et al.: "Effects of β-guanidinopropionic acid-feeding on the patterns of myosin isoforms in rat fast-twitch muscle" Pflugers Arch.-Eur.J.Physiol.430. 389-393 (1995)

Ren, J.-M., Y. Ohira 等人：“β-胍基丙酸喂养对大鼠快肌中肌球蛋白亚型模式的影响”Pflugers Arch.-Eur.J.Physiol.430。 389-393 (1995)