Relationship between the contents of high-energy phosphates and Regulation of muscular properties

高能磷酸盐含量与肌肉特性调节的关系

• 批准号: 24650407
• 负责人: OHIRA Yoshinobu
• 金额: $ 2.25万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24650407/
• 关键词: 骨格筋; 可塑機構; 遺伝子; タンパク質; クレアチンアナログ

It is known that the properties of skeletal muscles are regulated in response to exercise training, environment, nutrition, growth and development, and/or aging. The energy source for muscle contraction is adenosine triphosphate (ATP), which is synthesized through aerobic and anaerobic energy metabolism. It is also influenced by the content of creatine (AJP Cell Physiol, ’94; Jpn J Physiol, ’94 & ’95). However, the precise mechanism responsible for the regulation of contractile and metabolic properties of muscles is still unclear. Therefore, the current study was performed to investigate the effects of creatine depletion, by feeding creatine analogue, β-guanidinopropionic acid (β-GPA) on the properties of plantaris muscle of C57BL6 mice. Three weeks old male mice were separated into two groups randomly. One group of mice were fed regular powdered diet and other group were supplied the same diet, but containing 1% β-GPA. The amount of food, pair -fed, was gradually increased following growth, but 1 g of food was given to each mouse daily after 3 weeks. Water was supplied ad libitum. At 4th and 8th week, blood and muscle samplings were performed. Blood was withdrawn from the jugular vein under anesthesia with i.p. injection of sodium pentobarbital. Serum was saved after centrifugation and creatine content was analyzed. Plantaris muscles were sampled bilaterally. Muscle fiber types were analyzed in the cross-section of right muscles. Gene expressions were determined in the left muscles. Significant depletion of creatine and shift of fibers toward slow-type were noted, especially after 8 weeks of β-GPA feeding. Increase in the expression of genes related to slow-type fibers and mitochondrial metabolism was noted even at 4th week. It is suggested that depletion of creatine causes the transformation of muscle fibers toward slow-twitch type.

众所周知，骨骼肌的特性受运动训练、环境、营养、生长发育和/或衰老的影响。肌肉收缩的能量来源是三磷酸腺苷，它是通过有氧和无氧能量代谢合成的。它还受肌酸含量的影响(AJP Cell Physiol，‘94；JPn J Physiol，’94&‘95)。然而，调节肌肉收缩和代谢特性的确切机制仍不清楚。因此，本研究通过饲喂肌酸类似物β-GPA(β-GPA)来研究肌酸耗竭对C57BL6小鼠足底肌电特性的影响。将3周龄雄性小鼠随机分为两组。一组小鼠饲喂常规粉末状饲料，另一组饲喂相同的饲料，但添加1%的β-GPA。随着生长发育，配对喂养的食量逐渐增加，但在3周后，每只小鼠每天摄入1g食物。水是临时供应的。分别于第4周和第8周取血和肌肉标本。在静脉注射麻醉下从颈静脉取血。注射戊巴比妥钠。离心后保存血清，测定肌酸含量。分别于双侧足底肌取标本。对右肌横断面的肌纤维类型进行分析。测定左侧肌肉中的基因表达。特别是在β-GPA喂养8周后，肌酸显著减少，纤维向慢型转变。慢型纤维和线粒体代谢相关基因的表达在第4周仍有增加。提示肌酸耗竭导致肌纤维向慢抽动型转化。

项目成果

Evaluation of gene, protein and neurotrophin expression in the brain of mice exposed to space environment for 91 days.

• DOI: 10.1371/journal.pone.0040112
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Santucci D;Kawano F;Ohira T;Terada M;Nakai N;Francia N;Alleva E;Aloe L;Ochiai T;Cancedda R;Goto K;Ohira Y
• 通讯作者: Ohira Y

骨格筋の量的変化に伴う細胞内脂肪とその分布

细胞内脂肪及其分布与骨骼肌数量变化的关系

• 发表时间: 2012
• 作者: 土屋隆裕;大平友宇
• 通讯作者: 大平友宇

Regional Neural Activation Defines a Gateway for Autoreactive T Cells to Cross the Blood-Brain Barrier

• DOI: 10.1016/j.cell.2012.01.022
• 发表时间: 2012-02-03
• 期刊: CELL
• 影响因子: 64.5
• 作者: Arima, Yasunobu;Harada, Masaya;Murakami, Masaaki
• 通讯作者: Murakami, Masaaki

Biomedical analysis of rat body hair after hindlimb suspension for 14 days

• DOI: 10.1016/j.actaastro.2011.12.016
• 发表时间: 2012-04
• 期刊: Acta Astronautica
• 影响因子: 3.5
• 作者: M. Terada;F. Kawano;N. Ishioka;A. Higashibata;H. Majima;Takashi Yamazaki;T. Watanabe-Asaka;M. Niihori;Reiko Nakao;Shin Yamada;C. Mukai;Y. Ohira

The impact of long-term exposure to space environment on adult mammalian organisms: a study on mouse thyroid and testis.

• DOI: 10.1371/journal.pone.0035418
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Masini MA;Albi E;Barmo C;Bonfiglio T;Bruni L;Canesi L;Cataldi S;Curcio F;D'Amora M;Ferri I;Goto K;Kawano F;Lazzarini R;Loreti E;Nakai N;Ohira T;Ohira Y;Palmero S;Prato P;Ricci F;Scarabelli L;Shibaguchi T;Spelat R;Strollo F;Ambesi-Impiombato FS
• 通讯作者: Ambesi-Impiombato FS