Liquid Chromatography Coupled Hybrid Mass Spectrometer

液相色谱耦合混合质谱仪

• 批准号: 445432254
• 金额: --
• 依托单位: Karlsruher Institut für Technologie (KIT)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/445432254?language=en
• 关键词: Liquid; Chromatography; Coupled; Hybrid; Mass

In this grant proposal, we apply for an mass spectrometer to be used in the biological institutes (Institute for Applied Biosciences, Botanical Institute) at the Karlsruhe Institute of Technology (KIT). Applications comprise both analyses of metabolites (including (non-protein-)polymers) as well as proteomics applications. A hybrid quadrupole mass spectrometer, as a high-resolution, accurate-mass, sensitive and sufficiently fast scanning spectrometer, is the ideal device fulfilling the requirements as defined by the research areas of the participating working groups. The mass spectrometer is to be used, among other things, in shotgun proteomics of complex samples (food, cells and tissues, biological fluids), in the analysis of post-translational protein modifications, in structure elucidation of glycoconjugates (directly or after enzymatic hydrolysis) and plant secondary metabolites as well as for highly sensitive and accurate quantification approaches using targeted techniques (including stable isotope dilution assays). In order to create ideal conditions for analyses of metabolites as well as for proteomics applications, the mass spectrometer needs to be coupled to either an ultra-high performance liquid chromatography system (UHPLC) or a nano high performance liquid chromatograph (NanoH-PLC). The system is completed by ion sources including electrospray ionization (ESI) and atmospheric chemical ionization (APCI) probes and a nitrogen generator with compressor.

在这项拨款提案中，我们申请了一台质谱仪用于卡尔斯鲁厄理工学院（KIT）的生物研究所（应用生物科学研究所、植物研究所）。应用包括代谢物（包括（非蛋白质）聚合物）分析以及蛋白质组学应用。混合四极杆质谱仪作为一种高分辨率、精确质量、灵敏且足够快的扫描光谱仪，是满足参与工作组研究领域所定义要求的理想设备。除其他外，质谱仪可用于复杂样品（食品、细胞和组织、生物液体）的鸟枪蛋白质组学、翻译后蛋白质修饰分析、糖缀合物（直接或酶水解后）和植物次生代谢物的结构阐明，以及使用靶向技术（包括稳定 同位素稀释测定）。为了为代谢物分析以及蛋白质组学应用创造理想的条件，质谱仪需要与超高效液相色谱系统 (UHPLC) 或纳米高效液相色谱仪 (NanoHPLC) 结合。该系统由离子源组成，包括电喷雾电离 (ESI) 和大气化学电离 (APCI) 探头以及带压缩机的氮气发生器。