Liquid Chromatography Coupled Hybrid Mass Spectrometer

在这项拨款申请中，我们申请了一台质谱仪，用于卡尔斯鲁厄理工学院（KIT）的生物研究所（应用生物科学研究所，植物研究所）。应用包括代谢物（包括（非蛋白）聚合物）的分析以及蛋白质组学应用。混合四极杆质谱仪作为一种高分辨率、高质量、高灵敏度和足够快的扫描光谱仪，是满足各参与工作组研究领域要求的理想设备。除其他外，质谱仪将用于复杂样品（食物、细胞和组织、生物液体）的鸟枪蛋白质组学分析，翻译后蛋白质修饰分析，糖缀合物（直接或经酶水解）和植物次级代谢物的结构阐明，以及使用靶向技术（包括稳定同位素稀释测定）的高灵敏度和精确定量方法。为了为代谢产物分析和蛋白质组学应用创造理想的条件，质谱仪需要与超高高效液相色谱系统（UHPLC）或纳米高效液相色谱仪（NanoH-PLC）耦合。该系统由包括电喷雾电离（ESI）和大气化学电离（APCI）探针在内的离子源和带压缩机的氮气发生器完成。

In this grant proposal, we apply for an mass spectrometer to be used in the biological institutes (Institute for Applied Biosciences, Botanical Institute) at the Karlsruhe Institute of Technology (KIT). Applications comprise both analyses of metabolites (including (non-protein-)polymers) as well as proteomics applications. A hybrid quadrupole mass spectrometer, as a high-resolution, accurate-mass, sensitive and sufficiently fast scanning spectrometer, is the ideal device fulfilling the requirements as defined by the research areas of the participating working groups. The mass spectrometer is to be used, among other things, in shotgun proteomics of complex samples (food, cells and tissues, biological fluids), in the analysis of post-translational protein modifications, in structure elucidation of glycoconjugates (directly or after enzymatic hydrolysis) and plant secondary metabolites as well as for highly sensitive and accurate quantification approaches using targeted techniques (including stable isotope dilution assays). In order to create ideal conditions for analyses of metabolites as well as for proteomics applications, the mass spectrometer needs to be coupled to either an ultra-high performance liquid chromatography system (UHPLC) or a nano high performance liquid chromatograph (NanoH-PLC). The system is completed by ion sources including electrospray ionization (ESI) and atmospheric chemical ionization (APCI) probes and a nitrogen generator with compressor.