Ultra-performance-liquid chromatography - coupled tandem mass spectrometry (UPLC-MS/MS)

超高效液相色谱-串联质谱 (UPLC-MS/MS)

• 批准号: 465137452
• 金额: --
• 依托单位: Technische Universität Dresden
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/465137452?language=en
• 关键词: Ultra; performance; liquid; chromatography; coupled

In the present application, the procurement of an ultra-performance liquid chromatography-coupled tandem mass spectrometer (UPLC-MS / MS) is requested, which will be used for targeted, sensitive and quantitative analysis of small molecules in the context of biomedical research. The main topics are metabolism-based studies of innate immunity, neuroinflammation and inflammatory processes within the development of various (tumor-) diseases, the establishment of new biomarkers in endocrinological research and studies on bone metabolism. Furthermore, metabolic studies are planned as part of developmental-psychological research projects with regard to eating disorders. A major focus is the establishment of "Metabolic FLUX" methods, which, in addition to the quantification of specific metabolites, also provides information about the metabolic pathway kinetics. Due to the broad research topics mentioned above, a large number of analytical methods are necessary to analyze the metabolites of both central and secondary metabolic pathways. Such methods refer to sugar metabolism (glycolysis, pentose-phosphate pathway) and energy metabolism (citric acid cycle, fatty acid oxidation) as well as amino acids and biogenic polyamines, adrenal steroid hormones, catecholamine metabolites and biomarkers for oxidative stress. The necessity of these methods is described in the accompanying project descriptions. Regarding the biomarkers of oxidative stress in particular, it is anticipated that a successful development of such a method will generate a broad interest within other working groups at the Dresden Medical Faculty. In order to process the large number of samples from the research projects as described in other parts of this application as well as to develop new analytical methods that are necessary for the successful processing of the research projects, the anticipated annual use of the requested instrumentation is approximately 6000 hours.

在本申请中，要求采购超高效液相色谱-串联质谱仪（UPLC-MS / MS），其将用于生物医学研究背景下小分子的靶向、灵敏和定量分析。主要议题是基于代谢的先天免疫研究，各种（肿瘤）疾病发展中的神经炎症和炎症过程，内分泌研究中新生物标志物的建立和骨代谢研究。此外，还计划将代谢研究作为饮食失调方面的发展心理研究项目的一部分。一个主要的焦点是建立“代谢通量”方法，除了定量特定的代谢物，还提供有关代谢途径动力学的信息。由于上述广泛的研究主题，需要大量的分析方法来分析中枢和次级代谢途径的代谢物。这些方法涉及糖代谢（糖酵解、戊糖-磷酸途径）和能量代谢（柠檬酸循环、脂肪酸氧化）以及氨基酸和生物源多胺、肾上腺类固醇激素、儿茶酚胺代谢物和氧化应激的生物标志物。这些方法的必要性在所附的项目说明中说明。特别是关于氧化应激的生物标志物，预计这种方法的成功开发将在德累斯顿医学院的其他工作组中产生广泛的兴趣。为了处理本申请其他部分所述的研究项目中的大量样品，以及开发成功处理研究项目所需的新分析方法，预计所需仪器的年使用量约为6000小时。