Bioreactor with integrated mass-spectroscopy

具有集成质谱仪的生物反应器

• 批准号: 445484440
• 金额: --
• 依托单位: Eberhard Karls Universität Tübingen
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/445484440?language=en
• 关键词: Bioreactor; integrated; mass; spectroscopy

The group of Prof. Largus (Lars) Angenent is conducting research in the field of Environmental Biotechnology at the Centre for Applied Geosciences (ZAG) at the University of Tübingen. His group will move in the beginning of 2020, together with 13 other environmental research disciplines, into the Geo- und Umweltforschungszentrum, which is a new building on the university’s campus. Angenent focuses on three core topics that have the common goal of recovering carbon from industrial gases and from organic wastewater into microbially generated fuels or other valuable chemicals (e.g., medium-chain carboxylic acids). This includes the topics of syngas fermentation, power-to-x, and chain elongation. The use of bioreactors is essential for this research. So far, the group has specifically designed and adapted these bioreactors to the respective project requirements. However, a unique design is not necessary for systems biology research with genetically modified pure cultures (e.g., Clostridium ljungdahlii, Methanothermobacter thermautotrophicus) and isotopically labelled C-containing gases. Here it is necessary to, for example, validate in-silico generated molecular biological genome-scale metabolic models (GEM) by standardized cultivation experiments to evaluate and adapt the metabolic model. In addition, genetically modified strain will be tested to answer specific research questions about the metabolic pathways. In this way, microbial metabolic processes in the fermentation of C-containing gases can be elucidated and their efficiency maximized for future biotechnological applications. Essential for the systems biology research is the use of fully automatic flow meters, which allow the supply and removal of substrate (C-containing gas) as well as the substrate turnover to be kept constant (steady-state). To evaluate the performance of the bioreactors, it is necessary to create a complete carbon balance of the incoming and outgoing C-components. A coupling of the bioreactor to a gas analysis device, which analyzes and quantifies the incoming C-gas substrate, the outgoing residual gas as well as microbial reaction products (e.g., CH4) at extremely high temporal resolution, is indispensable for this. Here, a bioreactor system with control unit and four bioreactor vessels is requested. This system allows the operation of up to four independently controllable bioreactor vessels. In addition, a gas mass spectrometer is included, which quantitatively identifies the incoming and outgoing gas phase of the respective bioreactors as well as the corresponding microbial reaction products. The advantage of a gas mass spectrometer compared to alternative measuring methods (GC-MS, infrared or gas meter) is the fast and precise measurement of organic and inorganic gas components without costly instrument conversions.

Largus（Lars）Angenent教授小组正在蒂宾根大学应用地球科学中心进行环境生物技术领域的研究。他的团队将在2020年初与其他13个环境研究学科一起搬进Geo- und Umweltforschungszentrum，这是该大学校园的一座新建筑。Angenent专注于三个核心主题，其共同目标是从工业气体和有机废水中回收碳，使其成为微生物产生的燃料或其他有价值的化学品（例如，中链羧酸）。这包括合成气发酵，功率到x和链伸长的主题。生物反应器的使用对这项研究至关重要。到目前为止，该集团已经专门设计和调整这些生物反应器，以满足各自的项目要求。然而，独特的设计对于使用遗传修饰的纯培养物的系统生物学研究是不必要的（例如，杨氏梭菌（Clostridium ljungdahlii）、热自养甲烷热杆菌（Methanothermobacter thermautotrophicus））和同位素标记的含C气体。例如，有必要通过标准化培养实验验证计算机生成的分子生物学基因组规模代谢模型（GEM），以评估和调整代谢模型。此外，将对转基因菌株进行测试，以回答有关代谢途径的具体研究问题。通过这种方式，可以阐明含碳气体发酵中的微生物代谢过程，并使其效率最大化，以用于未来的生物技术应用。系统生物学研究的关键是使用全自动流量计，它允许供应和去除底物（含碳气体）以及底物周转保持恒定（稳态）。为了评估生物反应器的性能，有必要建立进出C组分的完整碳平衡。将生物反应器耦合到气体分析装置，所述气体分析装置分析和定量进入的C-气体底物、流出的残余气体以及微生物反应产物（例如，CH 4）在极高的时间分辨率，是必不可少的。 这里，需要具有控制单元和四个生物反应器容器的生物反应器系统。该系统允许操作多达四个独立可控的生物反应器容器。此外，还包括一个气体质谱仪，可定量识别各个生物反应器的进出气相以及相应的微生物反应产物。与其他测量方法（GC-MS、红外线或气量计）相比，气体质谱仪的优势在于可以快速、精确地测量有机和无机气体成分，而无需昂贵的仪器转换。