High Resolution Confocal Spinning Disc System

高分辨率共焦旋转盘系统

• 批准号: 447766904
• 金额: --
• 依托单位: Charité - Universitätsmedizin Berlin
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/447766904?language=en
• 关键词: Resolution; Confocal; Spinning; Disc; System

Spinning disk confocal (SDC) microscopy is one of the most successful imaging methods to investigate subcellular dynamics. The fast and sensitive camera based acquisition combined with a confocal mode enable researchers to perform multicolor 3D live-cell experiments over extended times. The AMBIO core facility currently has only one SDC system that has been successfully used in ~ 80 projects from research groups throughout Berlin. Not surprisingly, this system is frequently overbooked, limiting the planning of live-cell experiments. The lack of advanced features further prevents several groups from performing optimized imaging projects. Several high-end user groups (i.e. AGs Reber, Harms, Eickholt, Tacke, s. Supplement for Research Groups) who requested more usage capacities and advanced technical features are therefore part of this proposal to procure a novel SDC system that in addition to previous functionalities (multiplexing, automation) offers novel features (i.e. large field of view, optics for tissue imaging, super resolution) that enhance throughput and resolution. Beyond these groups the proposed system will be available to all AMBIO users (currently ~ 115 from ~ 42 research groups) to perform improved high-resolution live-cell imaging projects.

旋转圆盘共聚焦显微镜是研究亚细胞动力学最成功的成像方法之一。这种基于相机的快速而灵敏的采集与共焦模式相结合，使研究人员能够在更长的时间内进行多色3D活细胞实验。AMBIO核心设施目前只有一个SDC系统，该系统已在柏林各地研究小组的约80个项目中成功使用。不足为奇的是，这个系统经常超额预定，限制了活细胞实验的计划。高级功能的缺乏进一步阻碍了几个团队执行优化的成像项目。因此，几个高端用户群体(即AGs Reber、Harm、Eickholt、Tacke、S。研究组补充资料)要求更多的使用能力和先进的技术特征，因此是这项提议的一部分，目的是采购一种新型的SDC系统，该系统除了先前的功能(多路复用、自动化)外，还提供新的特征(即大视场、用于组织成像的光学设备、超分辨率)，以提高吞吐量和分辨率。在这些小组之外，所有AMBIO用户(目前是42个研究小组中的115个)都可以使用建议的系统来执行改进的高分辨率活细胞成像项目。