Spinning disk confocal system

神经病理学研究所、人类遗传学研究所、细胞生物学研究所联合申请双CMOS旋转盘共聚焦显微镜和集成frap激光器的固态烧蚀激光器。通过同时成像两个通道，现代双摄像头系统可以实时分析活细胞和生物体的亚细胞结构和过程，具有高分辨率。FRAP可以同时确定结构和过程的动力学。此外，显微镜允许将斑马鱼模型整合到RWTH的疾病机制分析中。烧蚀激光将允许调节亚细胞结构，例如，它将有可能确定调节细胞骨架或细胞延伸如轴突的后果。能够完成这些任务的共聚焦显微镜目前还不存在于医学院或亚琛工业大学。计划研究的主要重点是神经系统疾病，特别是周围神经和骨骼肌的遗传性疾病以及神经退行性疾病。申请人具有长期研究神经系统疾病和使用共聚焦显微镜的经验。这些研究的结果促成了一些高影响力的出版物，其中一些是与有关机构合作出版的。投资于旋转盘共聚焦系统是继续和扩大我们的项目的必要条件。它还将允许izkf联合研究项目“钠通道相关疼痛障碍”（人类遗传学和其他）和其他小组将时间和空间高分辨率的现代荧光共聚焦成像与亚细胞结构的体内激光操作相结合。

The Institute of Neuropathology, the Institute of Human Genetics, and the Institute of Cell Biology jointly apply for a Spinning disk confocal microscope with two CMOS cameras and a solid state ablation laser with integrated FRAP-laser. By simultaneously imaging two channels, the modern dual camera system allows real time analysis of subcellular structures and processes in living cells and organisms with high resolution. The dynamics of structures and processes can simultaneously be determined by FRAP. Furthermore, the microscope with allow to integrate the zebrafish model in the analysis of disease mechanisms at the RWTH. The ablation laser will allow to modulate subcellular structures, for example it will be possible to determine the consequences of modulating cytoskeleton or cellular extensions like axons. A confocal microscope that can fulfill these tasks does currently not exist at the medical faculty or the RWTH Aachen. The main focus of the planned studies will be on neurological diseases, especially hereditary diseases of peripheral nerves and skeletal muscle as well as neurodegenerative disorders. The applicants have longstanding experience in studying neurological diseases and in using confocal microscopy. The results of these studies have contributed to several high impact publications, some of them in collaborations of the involved institutions. Investing in the Spinning disk confocal system is necessary to continue and extend our projects. It will also allow the IZKF-joint research project „sodium channel-associated pain disorders“ (Human Genetics and others) and other groups to combine temporally and spatially highly resolved modern fluorescent confocal imaging combined with in vivo-laser manipulation of subcellular structures.