Artificial periodontal ligament with honey-comb structure using tissue engineering

利用组织工程的蜂窝状结构人工牙周膜

• 批准号: 14207093
• 负责人: INOUE Takashi
• 金额: $ 30.78万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14207093/
• 关键词: artificial periodontal ligament; tissue engineering; honey-comb structure; scaffold; plasma treatment; fibronectin; b FGF; implant; Sinvastatin; セラミックス; Tissue engineering; マラッセ上皮遺残; stem cell; ナノ形状制御; メカニカルストレス

The purpose of this project was to create the artificial periodontal ligament around the dental implant and periodontal defect using tissue engineering. To create the functional periodontal ligament might be necessary to provide following conditions : first, proliferation of organic stem cells around the dental implant or residual tooth and synthesize the collagen by these cells. Second, these proliferating cells have to differentiate into periodontal ligament fibroblast, cementblast or osteoblast depending on the surrounding situation, and finally these cells synthesis collagen fiber, cementum, or bone. First of all, we examined the osteogenic cell behavior on protein immobilized surface. Following plasma surface modification, fibronectin, fibroblast growth factor (FGF), and bone morphogenetic protein-2 (BMP) were immobilized. The number of osteogenic cells obtained from rat bone marrow adhering to fibronectin and FGF immobilized surface increased compared with control surface. Alkali … More ne phosphatase activity and osteocalcin mRNA expression of these cells on the BMP2 immobilized surface was greater than that of control groups. From these results, it is suggested that protein can be immobilized to a substratum after treatment with O2 plasma and that the osteogenic cells surrounding a biomaterial can be controlled by the immobilization of protein to the implant.Then we analyzed the quantity of new bone formed in vivo around calcium-immobilized titanium implants with surfaces modified using pamidronate (PAM), a nitrogen-containing bisphosphonate (N-BP), implants of pure titanium, and titanium implants immobilized with calcium ions. New bone formation was visualized using fluorescent labeling with intravenous injection. After 1 week, more new bone formed around the PAM-immobilized implant than around the calcium-immobilized and pure titanium implants. The was also seen with the new bone formation after 3 weeks. After 4 weeks, significantly more new bones were formed around the BP-immobilized implant than around the calcium ion-implanted and pure titanium implants. The new N-BP-modified titanium surface stimulates new bone formation around the implant, which might contribute to the success of implant therapy. Furthermore, immobilization of SVA onto dental implants using O2 plasma treatment resulted to promote osteogenesis at thae bone tissue/implant interface.These findings suggested that oxygen plasma modificantion was able to immobilize the proper proteins on the titanium implant, and contribute to proliferate the periodontal ligament like tissue. Less

本课题旨在利用组织工程技术在牙种植体周围及牙周缺损处构建人工牙周膜。为了创造功能性牙周膜，可能需要提供以下条件：首先，在牙种植体或残牙周围增殖有机干细胞，并由这些细胞合成胶原。其次，这些增殖的细胞必须根据周围情况分化成牙周膜成纤维细胞、成牙骨质细胞或成骨细胞，最后这些细胞合成胶原纤维、牙骨质或骨。首先，我们研究了成骨细胞在蛋白质固定化表面上的行为。等离子体表面改性后，纤维连接蛋白，成纤维细胞生长因子（FGF）和骨形态发生蛋白-2（BMP）被固定。从大鼠骨髓中获得的成骨细胞粘附到纤维连接蛋白和FGF固定化表面的数量与对照表面相比增加。碱 ...更多信息 BMP-2固定表面的细胞的磷酸酶活性和骨钙素mRNA表达均高于对照组。从这些结果，这是建议，蛋白质可以被固定到一个基层后，与O2等离子体处理和周围的成骨细胞的生物材料可以通过固定的蛋白质的implantation.Then我们分析了周围的钙固定钛种植体在体内形成的新骨的数量与表面改性帕米膦酸盐（PAM），含氮的双膦酸盐（N-BP），纯钛植入物和用钙离子固定的钛植入物。通过静脉注射荧光标记观察新骨形成。1周后，PAM固定的种植体周围形成的新骨比钙固定和纯钛种植体周围形成的新骨更多。3周后也可见新骨形成。4周后，BP固定种植体周围形成的新骨明显多于钙离子植入种植体和纯钛种植体周围。新的N-BP改性钛表面刺激种植体周围的新骨形成，这可能有助于种植体治疗的成功。O2等离子体处理后，SVA固定在种植体表面，促进了种植体/骨组织界面的成骨，表明O2等离子体处理后，SVA固定在种植体表面，有利于牙周膜样组织的增殖。少

项目成果

分子プレカーサーを用いたチタン基板上へのアパタイト薄膜形成-擬似体液内でのリン酸カルシウム生成-

使用分子前驱体在钛基底上形成磷灰石薄膜-在模拟体液中生产磷酸钙-

• 发表时间: 2002
• 期刊: 歯科材料・器械 24.1
• 作者: 高橋健一;早川徹;原広樹;吉成正雄;望月千尋;佐藤光史;根本君也
• 通讯作者: 根本君也

Ikeda, H., Shiraiwa, M., Yamaza, T., Inoue T, et al.: "Defference in penetration of horseradish peroxidase tracer a s foreign substance into th eperi-implant or junctional epitheliumof rat gingivae"Clinical Oral Implant Research. 13. 243-251 (2002)

Ikeda, H., Shiraiwa, M., Yamaza, T., Inoue T, et al.：“辣根过氧化物酶示踪剂作为异物渗透到大鼠牙龈种植体上皮或交界上皮中的差异”临床口腔种植体研究。

Characerization of the peri-implant epithelium in hamster palatine mucosa : Behavior of Merkel cells and nerve endings

仓鼠腭粘膜种植体周围上皮的表征：默克尔细胞和神经末梢的行为

• 发表时间: 2005
• 期刊: Biomedical Research 26(6)
• 作者: Yuta Suzuki;Kenichi Matsuzaka;Ken Ishizaki;Masakazu Tazaki;Toru Soto;Takashi Inoue.
• 通讯作者: Takashi Inoue.

Quartz crystal microbalance-dissipation technique for the study of initial adsorption of fibronectin onto tresvl chloride-activated titanium.

石英晶体微天平耗散技术用于研究纤连蛋白在三氯乙烯活化钛上的初始吸附。

• 发表时间: 2005
• 期刊: J Biomed Mater Res 77B・2
• 作者: Hayakawa T;Yoshinari M;Nemoto K
• 通讯作者: Nemoto K

Quartz crystal microbalance-dissipation technique for the study of initial adsorption of fibronectin onto tresyl chloride-activated titanium.

石英晶体微天平耗散技术用于研究纤连蛋白在三乙磺酰氯活化钛上的初始吸附。

• 发表时间: 2005
• 期刊: J Biomed Mater Res 73B-2
• 作者: Hayakawa T;Yoshinari M;Nemoto K
• 通讯作者: Nemoto K