Artificial periodontal ligament with honey-comb structure using tissue engineering

本课题旨在利用组织工程技术在牙种植体周围及牙周缺损处构建人工牙周膜。为了创造功能性牙周膜，可能需要提供以下条件：首先，在牙种植体或残牙周围增殖有机干细胞，并由这些细胞合成胶原。其次，这些增殖的细胞必须根据周围情况分化成牙周膜成纤维细胞、成牙骨质细胞或成骨细胞，最后这些细胞合成胶原纤维、牙骨质或骨。首先，我们研究了成骨细胞在蛋白质固定化表面上的行为。等离子体表面改性后，纤维连接蛋白，成纤维细胞生长因子（FGF）和骨形态发生蛋白-2（BMP）被固定。从大鼠骨髓中获得的成骨细胞粘附到纤维连接蛋白和FGF固定化表面的数量与对照表面相比增加。碱 ...更多信息 BMP-2固定表面的细胞的磷酸酶活性和骨钙素mRNA表达均高于对照组。这些结果表明，用O2等离子体处理后，蛋白质可以固定在基底上，并且可以通过将蛋白质固定在植入物上来控制生物材料周围的成骨细胞。然后，我们分析了钙固定钛植入物周围体内形成的新骨数量，这些植入物的表面使用帕米膦酸盐（PAM）（一种含氮双磷酸盐（N-BP））进行修饰，纯钛植入物和用钙离子固定的钛植入物。通过静脉注射荧光标记观察新骨形成。1周后，PAM固定的种植体周围形成的新骨比钙固定和纯钛种植体周围形成的新骨更多。3周后也可见新骨形成。4周后，BP固定种植体周围形成的新骨明显多于钙离子植入种植体和纯钛种植体周围。新的N-BP改性钛表面刺激种植体周围的新骨形成，这可能有助于种植体治疗的成功。O2等离子体处理后，SVA固定在种植体表面，促进了种植体/骨组织界面的成骨，表明O2等离子体处理后，SVA固定在种植体表面，有利于牙周膜样组织的增殖。少

The purpose of this project was to create the artificial periodontal ligament around the dental implant and periodontal defect using tissue engineering. To create the functional periodontal ligament might be necessary to provide following conditions : first, proliferation of organic stem cells around the dental implant or residual tooth and synthesize the collagen by these cells. Second, these proliferating cells have to differentiate into periodontal ligament fibroblast, cementblast or osteoblast depending on the surrounding situation, and finally these cells synthesis collagen fiber, cementum, or bone. First of all, we examined the osteogenic cell behavior on protein immobilized surface. Following plasma surface modification, fibronectin, fibroblast growth factor (FGF), and bone morphogenetic protein-2 (BMP) were immobilized. The number of osteogenic cells obtained from rat bone marrow adhering to fibronectin and FGF immobilized surface increased compared with control surface. Alkali … More ne phosphatase activity and osteocalcin mRNA expression of these cells on the BMP2 immobilized surface was greater than that of control groups. From these results, it is suggested that protein can be immobilized to a substratum after treatment with O2 plasma and that the osteogenic cells surrounding a biomaterial can be controlled by the immobilization of protein to the implant.Then we analyzed the quantity of new bone formed in vivo around calcium-immobilized titanium implants with surfaces modified using pamidronate (PAM), a nitrogen-containing bisphosphonate (N-BP), implants of pure titanium, and titanium implants immobilized with calcium ions. New bone formation was visualized using fluorescent labeling with intravenous injection. After 1 week, more new bone formed around the PAM-immobilized implant than around the calcium-immobilized and pure titanium implants. The was also seen with the new bone formation after 3 weeks. After 4 weeks, significantly more new bones were formed around the BP-immobilized implant than around the calcium ion-implanted and pure titanium implants. The new N-BP-modified titanium surface stimulates new bone formation around the implant, which might contribute to the success of implant therapy. Furthermore, immobilization of SVA onto dental implants using O2 plasma treatment resulted to promote osteogenesis at thae bone tissue/implant interface.These findings suggested that oxygen plasma modificantion was able to immobilize the proper proteins on the titanium implant, and contribute to proliferate the periodontal ligament like tissue. Less