Microbiological environmental assessment in Southeast Asia

东南亚微生物环境评估

• 批准号: 15201006
• 负责人: NASU Masao
• 金额: $ 31.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15201006/
• 关键词: Southeast Asia; Aquatic environment; Microbes; Environmental evaluation; New methods; tet genes; Gene transfer; Algae; サスペンションアレイ; 藍藻; 遺伝子; 群集構造; 病原細菌; DNAマイクロアレイ; マイクロコズム

Objective of this research project is to assure microbiological quality of fresh water in order to keep safety of aquatic environment in Southeast Asia.Research results in FY20051.DNA microarray with newly designed probes was created. It consists of the oligonucleotide probes which corresponds to the 16S rRNA of bacteria. This DNA microarray provided us to obtain a convenient approach to detect various species of bacteria in aquatic environment simultaneously.2.According to the research, bacterial physiological states can be estimated by fluorescence in situ hybridization with vital staining and quantification of mRNA of gyrB gene.3.The frequencies of occurrence of tetracycline-resistant bacteria were higher in samples obtained at the sites in Kracheh (Cambodia) and Can Tho (Viet Nam) along the Mekong River than the sites in the lake Tonle Sape (Cambodia) and the Ho Chi Minh estuarine (Viet Nam). Various tetracycline genes were found in sediment of the Mekong River.4.The extracellular metabolic products by Microcystis aeruginosa enhanced conjugal transfer of plasmid.

本课题旨在确保淡水微生物质量，以保证东南亚地区水环境的安全。20051年度研究成果。用新设计的探针创建了DNA微阵列。它由与细菌16S rRNA相对应的寡核苷酸探针组成。这种DNA芯片为我们同时检测水生环境中多种细菌提供了一种方便的方法。根据研究，通过荧光原位杂交活体染色和gyrB基因mRNA的定量，可以估计细菌的生理状态。在湄公河沿岸的柬埔寨克拉切和越南芹苴的样本中，四环素耐药菌的出现频率高于柬埔寨洞里湖和越南胡志明河口的样本。在湄公河沉积物中发现了多种四环素基因。铜绿微囊藻胞外代谢产物促进质粒的偶联转移。

项目成果

A combination of direct viable counting, fluorescence in situ hybridization and green fluorescent protein gene expression for estimating plasmid transfer at the single level

结合直接活菌计数、荧光原位杂交和绿色荧光蛋白基因表达，用于单水平估计质粒转移

• 发表时间: 2006
• 期刊: Microbes and Environments (in press)
• 作者: Jorquera;M. et al.
• 通讯作者: M. et al.

Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites

• DOI: 10.1016/j.femsle.2004.06.026
• 发表时间: 2004-08-01
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Kim, SR;Nonaka, L;Suzuki, S
• 通讯作者: Suzuki, S

The enhancement of conjugal plasmid pHBR1 transfer between bacteria in the presence of extracellular metabolic products produced by Mycrocystis aeruginosa

铜绿脓肿产生的细胞外代谢产物存在下，增强细菌间接合质粒 pHBR1 的转移

• 发表时间: 2004
• 期刊: EMS Microbiology Ecology 51
• 作者: Ueki;M.;Matsui;K.;Choi;K.;Kawabata;Z.
• 通讯作者: Z.

Simultaneous enumeration of viable Enterobacteriaceae and Pseudomonas within three hours by multicolor fluorescence in situ hybridization with vital staining

通过活体染色多色荧光原位杂交在三小时内同时计数活肠杆菌和假单胞菌

• 发表时间: 2006
• 期刊: Journal of Microbiological Methods 65
• 作者: Kitaguchi;A.;et al.
• 通讯作者: et al.

Development of phylogenetic oligonucleotide probes for screening foodborne bacteria

开发用于筛查食源性细菌的系统发育寡核苷酸探针

• 发表时间: 2005
• 期刊: Journal of Health Science 51
• 作者: Ikeda;M. et al.
• 通讯作者: M. et al.