BIOLOGYCAL ESTIMATION OF AGUATIC ENVIRONMENT IN SOUTHEAST ASIA BYDNA MICROARRAY

BYDNA微阵列对东南亚水环境的生物评价

• 批准号: 13376003
• 负责人: NASU Masao
• 金额: $ 21.8万
• 依托单位: OSAKA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13376003/
• 关键词: southeast Asia; pollution; aquatic environment; bacteria; algae; fish; community strurture; gene expression; DNAチップ; 河川; 原核生物; 真核生物; タイ; 化学物質; 重金属; 水圏環境

In order to understand aquatic environments in Southeast Asia biologically, we performed development of DNA microarray for analyzing bacterial community structure and identification of environmental stress response genes of fish and alga.1. Bacterial community structures in rivers and canals in Bangkok were analyzed, and dominant bacterial groups were identified.2. Changes and similarities of bacterial community structures in a microcosm were monitored by DNA microarry targeting 16S ribosomal RNA genes.3. Expression of the metallothionein gene in the fish culture cells was induced by treatment with cadmium, zinc, arsenic, or t-butyl hydroperoxide. The expression level was depend on their concentration and treatment time.4. Expression of the heat shock protein gene (hsp70) in the fish culture cells was induced by arsenic treatment.5. Phytochelatin synthesis was induced strongly, by activation of glutathione synthesis pathway with heavy metal treatment in marine green alga, Dunal3ella tertlofecta.6. Phytochelatin can reduce the oxidation stress caused by various environmental stresses in Dunaliella tertiolecta.

为了从生物学上了解东南亚的水生环境，我们开发了DNA芯片，用于分析细菌群落结构和鉴定鱼和藻类的环境应激反应基因。对曼谷地区河流和运河的细菌群落结构进行了分析，确定了优势菌群。利用靶向16S核糖体RNA基因的DNA微载技术，监测微观环境下细菌群落结构的变化和相似性。镉、锌、砷和过氧化氢叔丁基处理均可诱导鱼培养细胞中金属硫蛋白基因的表达。表达量与它们的浓度和处理时间有关。砷处理可诱导鱼培养细胞表达热休克蛋白基因（hsp70）。重金属处理激活了海洋绿藻（Dunal3ella tertlofecta）的谷胱甘肽合成途径，强烈地诱导了植物螯合素的合成。植物螯合素可以降低杜氏藻因各种环境胁迫引起的氧化应激。

项目成果

Yoshida, K. et al.: "Induction of tolerance to oxidative stress in the green alga, Chlamydomonas reinhardtil, by abscisic acid."Plant Cell Envrion.. 26. 451-457 (2003)

Yoshida, K. 等人：“通过脱落酸诱导绿藻莱茵衣藻对氧化应激的耐受性。”Plant Cell Envrion.. 26. 451-457 (2003)

Tsuji, N. et al.: "Photosynthesis-dependent removal of 2,4-dichlorophenol by Chlorella fusca var, vacuolata."Biotechnol. Lett.. 25. 241-244 (2003)

Tsuji, N. 等人：“Chlorella fusca var, vacuolata 依靠光合作用去除 2,4-二氯苯酚。”生物技术。

Kimura, T. et al.: "Sensitivity of methallothionein-null mice to LPS/D-galactosamine-induced lethality."Biochem. Biophys. Res. Commun.. 280. 358-362 (2001)

Kimura, T. 等人：“甲硫氨酸缺失小鼠对 LPS/D-半乳糖胺诱导致死的敏感性。”Biochem。

V.Cheevaporn et al.: "Trend in lead content of airborne particles and mass of PM10 in metropolitan Bankok."J.Health Sci.. 50. 86-91 (2004)

V.Cheevaporn 等人：“Bankok 大都市空气中颗粒物的铅含量和 PM10 质量的趋势。”J.Health Sci.. 50. 86-91 (2004)

Tsuji, N. et al.: "Regulation of phytochelatin synthesis by zinc and cadmium in Dunaliella tertiolecta."Phytochem.. 62. 453-459 (2003)

Tsuji, N. 等人：“Dunaliella tertiolecta 中锌和镉对植物螯合素合成的调节。”Phytochem.. 62. 453-459 (2003)