BIOLOGYCAL ESTIMATION OF AGUATIC ENVIRONMENT IN SOUTHEAST ASIA BYDNA MICROARRAY

为了从生物学上了解东南亚的水环境，我们开展了用于分析细菌群落结构和鉴定鱼类和藻类环境应激反应基因的DNA微阵列的研制。结果1.分析了曼谷地区河流和运河的细菌群落结构，确定了优势细菌类群。利用针对16S核糖体RNA基因的DNA微阵列技术监测微生物群落结构的变化和相似性。金属硫蛋白基因在鱼类培养细胞中的表达被镉、锌、砷或叔丁基氢过氧化氢诱导。它们的表达水平取决于它们的浓度和处理时间。砷诱导鱼类培养细胞热休克蛋白基因(HSP70)的表达。重金属处理对海洋绿藻Dunal3ella tertloecta.的谷胱甘肽合成途径有较强的诱导作用。植物螯合素可以减轻杜氏藻因各种环境胁迫引起的氧化胁迫。

In order to understand aquatic environments in Southeast Asia biologically, we performed development of DNA microarray for analyzing bacterial community structure and identification of environmental stress response genes of fish and alga.1. Bacterial community structures in rivers and canals in Bangkok were analyzed, and dominant bacterial groups were identified.2. Changes and similarities of bacterial community structures in a microcosm were monitored by DNA microarry targeting 16S ribosomal RNA genes.3. Expression of the metallothionein gene in the fish culture cells was induced by treatment with cadmium, zinc, arsenic, or t-butyl hydroperoxide. The expression level was depend on their concentration and treatment time.4. Expression of the heat shock protein gene (hsp70) in the fish culture cells was induced by arsenic treatment.5. Phytochelatin synthesis was induced strongly, by activation of glutathione synthesis pathway with heavy metal treatment in marine green alga, Dunal3ella tertlofecta.6. Phytochelatin can reduce the oxidation stress caused by various environmental stresses in Dunaliella tertiolecta.