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Research on long distance movement of bacteria by Asian dust

亚洲沙尘对细菌的长距离移动研究

基本信息

批准号：
17256001
负责人：
NASU Masao
金额：
$ 27.04万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17256001/
关键词：
Asian dust Bacteria Dvnamics Environmental microbiology Molecular microbial ecology Loess Plateau Taklamakan desert

项目摘要

"Asian Dust" is the transportation of a large amount of soil particles originated from arid regions in China and Mongolia such as Taklamakan desert, Gobi desert and Loess plateau, and these particles are transported to East Asia (China, Korea and Japan) by the prevailing westerly winds. In Japan, more than one million tons of dust particles are estimated to fall on ground per year (reported by the National Institute for Environmental Studies, Japan). We supposed that these soil particles could be carriers of microbes. In order to clarify the dynamics of microbes moving with these dust events, bacterial cells attached to dust particles were detected by culture-independent approaches.(1) Particle size distribution was measured by scanning electron microsrepic (SEM) image analysis. Dust samples collected in Beijing and Osaka were below 10μm and 5μm in diameter, respectively, and they are thought to be carriers of microbes.(2) Particle characteristics were examined by SEM equipped with an energy-dispersive X-ray analyzer (SEM-EDX). The dust samples collected in Beijing and Osaka contained many small particles with distinctive soil characteristics.(3) New technique to visualize the bacterial cells on particles by ribosomal RNA-targeted approach and scanning electron microscopy (SEM-ISH) was developed.(4) By SEM-ISH analysis, bacterial cells attached on Asian Dust particles collected in Beijing were directly visualized. This indicated that bacteria can "travel" with "Asian Dust".(5) Amount of microbes moving with Asian dust was determined by real time PCR. The voltune of bacterial cells on Asian dust particles to Beijing estimated as 10^<13>～^<16> cells/month/km^2 in spring season.(6) Newly fabricated dust sampler was developed and the dust particles at the altitude of 1000 to 2000m in Japan were collected by a small airplane to avoid contamination of soil particles from ground.

“亚洲沙尘”是大量源自中国和蒙古干旱地区的土壤颗粒，如塔克拉玛干沙漠、戈壁沙漠和黄土高原，这些颗粒被盛行的西风输送到东亚（中国、韩国和日本）。在日本，估计每年有100多万吨尘埃颗粒落在地面上（日本国家环境研究所报告）。我们认为这些土壤颗粒可能是微生物的载体。为了阐明这些尘埃事件中微生物的动态，通过非培养方法检测附着在尘埃颗粒上的细菌细胞。(1)通过扫描电子显微镜（SEM）图像分析测量粒度分布。在北京和大坂采集的灰尘样品直径分别小于10μm和5μm，它们被认为是微生物的载体。(2)通过配备有能量分散X射线分析仪（SEM-EDX）的SEM检查颗粒特性。在北京和大坂收集的灰尘样品中含有许多具有独特土壤特征的小颗粒。(3)利用核糖体RNA靶向技术和扫描电子显微镜（SEM-ISH）技术，在颗粒上观察细菌细胞。(4)通过SEM-ISH分析，直接观察到了北京亚洲沙尘颗粒物上的细菌细胞。这表明细菌可以与“亚洲尘埃”一起“旅行”。(5)通过真实的时间PCR测定随亚洲灰尘移动的微生物的量。春季亚洲沙尘颗粒物上细菌细胞的数量为10^<13>^ cells<16>/month/km^2。(6)研制了一种新型粉尘采样器，并利用小型飞机采集了日本海拔1000 ~ 2000 m的粉尘颗粒，避免了地面土壤颗粒的污染。

项目成果

期刊论文数量（46）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Rapid identification and enumeration of antibiotic resistant bacteria in urban canals by microcolony-fluorescence in situ hybridization

DOI：
10.1248/jhs.52.703
发表时间：
2006-12-01
期刊：
JOURNAL OF HEALTH SCIENCE
影响因子：
0
作者：
Kenzaka, Takehiko;Yamaguchi, Nobuyasu;Nasu, Masao
通讯作者：
Nasu, Masao

rRNA 基因靶向方法确定亚洲尘埃与其源区细菌群落结构的相似性。

DOI：
发表时间：
2010
期刊：
Microbes Environ.
影响因子：
0
作者：
Y. Nishimura;et al.
通讯作者：
et al.

Application of real-time long and short polymerase chain reaction for sensitive monitoring of the fate of extracellular plasmid DNA introduced into river waters

应用实时长短聚合酶链反应灵敏监测引入河水中的细胞外质粒 DNA 的命运

DOI：
发表时间：
2008
期刊：
Microbes and Environments 23
影响因子：
0
作者：
F.Maruyama;et al.
通讯作者：
et al.

Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system

使用基于微流控芯片的系统通过微珠测定快速、简单地检测食物中毒细菌

DOI：
发表时间：
2006
期刊：
Microbiol.Methods 67
影响因子：
0
作者：
M.Ikeda;et al.
通讯作者：
et al.

Comparison of microbial community structure in Asian dust and its source region targeted 16S rRNA gene between by T-RFLP

T-RFLP比较亚洲沙尘及其源区16S rRNA基因微生物群落结构

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
T.Kenzaka;et al.;馬場貴志;T.Baba;馬場貴志;T.Baba;馬場貴志;T.Baba;馬場貴志;T.Baba;那須正夫;M.Nasu;山口進康;N.Yamaguchi;N. Yamaguchi;馬場貴志;T.Baba;山口進康;馬場貴志;T.Baba;N.Yamaguchi;末吉祥朗;A.Sueyoshi;馬場貴志;T.Baba;山口進康;N.Yamaguchi;M.Nasu;M.Nasu;末吉祥朗;西村義則;Y.Nishimura
通讯作者：
Y.Nishimura