The analysis of ERECTA gene, which is responsible for the elongation of the stem, in Arabidopsis.

本研究旨在了解高等植物的发育过程，特别是拟南芥花茎的伸长过程。为此，我们的工具是分离突变体并分析起作用的基因。两种勃起结合蛋白基因的研究。erbpl和2个双突变体表现出幼苗致死性。通过对胚胎从球形期到成熟期的观察，寻找个体发育缺陷点。心脏期胚胎发育后的缺陷。利用生长素报告基因dr5 - uida测定其与生长素的关系。寻找ERECTA基因调控的新因子。利用ER- uida（来自大肠杆菌）报告基因收集了许多ER基因表达降低的突变体。我们分离了erecta (as rex) 1和2基因表达降低的突变体。它们负责的基因分别编码果胶合成和酒窖细胞器的因子。在rexl和2背景下测定相关基因的调控情况，了解其调控作用。

The aim of this study is: to understand the processes during the development of higher plants especially for the elongation of the floral stems in Arabidopsis. For that, our tools are to isolate mutants and to analyze the responsible genes.1.The study of two genes for erecta-binding proteins.The erbpl and 2 double mutants expressed seedling-lethality. The ontogenic defective point was searched by observation of the embryos from globular stage to maturity. The defect revealed after the onset of the heart-stage-embryo. The relation to auxin was determined by using auxin-reporter DR5-uidA.2.To search new factors for the regulation of ERECTA gene.We collected many mutants which have reduced expression of ER gene by using ER-uidA(from E. coli) reporter. We isolated mutants carrying reduced expression of erecta (as rex) 1 and 2 genes. Their responsible genes encode for pectin-synthesis and for factors for cellar organella, respectively. The regulation of related genes were determined under the rexl and 2 background to know the regulation.