The analysis of the development of reproductive (male and female) organs

生殖（男性和女性）器官发育分析

• 批准号: 07281101
• 负责人: KOMEDA Yoshibumi
• 金额: $ 199.55万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-07281101/
• 关键词: development of inflorescences; pollen; Y-chromosome; floral meristem; male sterile; de-differentiation; liverworts; pollen mother cells; ホメオティック突然変異; 融合タンパク質; 分脱化; 雄雌不稔性; 花序形成; 器官形成遺伝子; イネ花器官数; アサ性染色体; アブラナカルシウム結合タンパク質遺伝子; テンサイ優性不稔; タバコ脱

We have studied the analyses of the development of sex-organes in order to analyze the reproductive systems of plants.At first, DNA structures and functions of sex-chromosomes have been studies in hemp and liverworts as experimental materials. And then, genetic networks, regulations and interactions have been studied for the development of floral organes in monocots and dicots. The analyses have been extended to the study of pollens. The research in pollens included the genetic regulation of male-sterility, the pollen-maturation-genes, and de-differentiation of immature pollens.In order to carry out the overall research project, individual research was collected and reviewed with each other. Thus, the project was developed as a whole.Individual studies were summaried as follows.1) The PDF1 gene responsible for the development of inflorescences has been analysed thoroughly.2) The dadl and mam genes have been analyzed. This study analyzed the drought tolerance of pollens and the guidance of pollen-tube-growth.3) The developmental mutants in rice have been studies. The mutants are responsible for the shape of ear, floral meristem, and regulation of the number of organes.4) Y-chomosome in hemp is special sequence and is rich with retrotransposons.5) The Brassica genes have been analyzed for pollen-specific Ca++-binding proteins and surface proteins of pollens.6) The genes responsible for the male-sterility have been analyzed in beats.7) Extensive study of expressed genes has been performed during the de-differentiation of tobacco pollens.8) The Y-choromosome in liverworts has been extensively analyzed and shown that this region has repetitive DNA regions.9) The lilly genes for cetromere-proteins and histone-like proteins have been analyzed.10) The functions of meiosis-specific LIM genes in pollen-mother-cells of lilly have been studied and speculated.

为了分析植物的生殖系统，我们研究了性器官发育的分析。首先，以大麻和苔类植物为实验材料，研究了性染色体的DNA结构和功能。在此基础上，研究了单子叶和双子叶花器官发育的遗传网络、调控和相互作用。这种分析已扩展到对花粉的研究。对花粉的研究包括雄性不育的遗传调控、花粉成熟基因和未成熟花粉的去分化。为了开展整体的研究项目，我们收集了个人的研究成果，并相互审查。因此，该项目是作为一个整体开发的。个别研究总结如下。1)深入分析了与花序发育有关的PDF1基因。对父系和母系基因进行了分析。本研究分析了花粉的耐旱性及对花粉管生长的指导作用。3)水稻发育突变体的研究。这些突变体对果穗的形状、花的分生组织和器官数量的调节负责。4)大麻的y染色体是一个特殊的序列，含有丰富的反转录转座子。5)对芸苔属植物花粉特异性钙结合蛋白和花粉表面蛋白进行了分析。对雄性不育的基因进行了分析。烟草花粉去分化过程中表达的基因已被广泛研究。8)对地精的y染色体进行了广泛的分析，发现该区域具有重复的DNA区域。9)对西曲粒蛋白和组蛋白样蛋白的lilly基因进行了分析。10)对百合花粉母细胞中减数分裂特异性LIM基因的功能进行了研究和推测。

项目成果

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Tozuka, A.：“通过线粒体 DNA 的 RFLP 分析揭示日本大豆的复合和临床分布”理论与应用遗传学。

Tanaka,I.: "A novel histone variant localized in nucleoli of higher plant cells." Chromosoma. (in press). (1990)

Tanaka,I.：“一种位于高等植物细胞核仁中的新型组蛋白变体。”

Nagasawa, N.: "DL regulates both leaf and pistil development in rice." Rice Genet. Newslett.(in press).

Nagasawa, N.：“DL 调节水稻叶片和雌蕊的发育。”

Toriyama, K.: "A cDNA clone encoding an lgE-binding protein from Brassica snther has significant sequence aimilarity to Ca^<2+>-binding proteins." Plant Mol Biol. (In press). (1996)

Toriyama, K.：“编码来自芸苔属 sther 的 lgE 结合蛋白的 cDNA 克隆与 Ca^2 结合蛋白具有显着的序列相似性。”

Nagai, J.: "Expressed sequence tags from immature female sexual organ of a liverwort, Marchantia polymorpha"DNA Research. 6. 1-11 (1999)

Nagai, J.：“来自地钱、地钱的未成熟雌性性器官的表达序列标签”DNA 研究。