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Genetic dissection of the development of the inflorescences in Arabidospsis thaliana.

拟南芥花序发育的遗传解剖。

基本信息

批准号：
07404056
负责人：
KOMEDA Yoshibumi
金额：
$ 3.97万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

The developmental mutations of Arabidopsis thaliana in erecta and acaulis5 have been analyzed molecular genetically in order to understand the developmental processes in the floral morphogenesis.At first, the ERECTA gene has been studied. From the studies carried out last year, we found the important cis-region for the expression of the ERECTA gene and constructed the transgenic plants expressing the reporter of the uidA gene with the cis-elements.We extented the analysis of these transgenic plants for the pattern of the expession using in situ techniques.For the analysis of trans-factors, we used yeast expression systems. The ERECTA-5' region for regulated expression was fused to the E.coli lacZ gene and the reporter was introduced into yeast cells. Second plasmids carrying plant cDNAs with yeast promoter were introduced into the yeast cells with the ERECTA-lacZ reporter. After screening 3,000,000 colonies, we found 11 cDNAs for the expression of the reporter. One clone was isolated and has been shown to have same expression-chacteristics and DNA homology to DNA-transcription factors.Secondly, we extended the study of acaulis5 mutations. Our strategy is the map-based gene walking for the isolation of the ACAULIS5 gene. As an initial stetp, physical mapping was further extended in the chromosome 5 upper (south) -arm. We idetified the contigs using DNA clones included in the P1 phage-plasmids. One P1 clone is the possible candidate for the hit.

为了了解拟南芥在花形态发生中的发育过程，从分子遗传学的角度分析了拟南芥在直立体和无茎系中的发育突变。首先，对ERECTA基因进行了研究。从去年开展的研究中，我们发现了ERECTA基因表达的重要顺式区域，构建了带有顺式元件的表达uidA基因报告基因的转基因植株，并利用原位技术对这些转基因植株的表达模式进行了分析。将调控表达的ERECTA-5‘区与E.ColiLacZ基因融合，并将报告基因导入酵母细胞。以ERECTA-LacZ为报告基因，将带有酵母启动子的植物cDNA载体导入酵母细胞。在筛选了300万个克隆后，我们发现了11个表达该记者的cDNA。分离到一个克隆，其表达特征和DNA同源性与DNA转录因子相同。第二，我们扩展了对acaulis5突变的研究。我们的策略是基于图谱的基因行走来分离ACAULIS5基因。作为最初的STETP，物理作图在5号染色体上(南)臂进一步延伸。我们使用包括在P1噬菌体质粒中的DNA克隆来鉴定重叠群。一个P1克隆是可能的热门候选。