Genetic dissection of the development of the inflorescences in Arabidospsis thaliana.

点击翻译按钮获取中文摘要

The developmental mutations of Arabidopsis thaliana in erecta and acaulis5 have been analyzed molecular genetically in order to understand the developmental processes in the floral morphogenesis.At first, the ERECTA gene has been studied. From the studies carried out last year, we found the important cis-region for the expression of the ERECTA gene and constructed the transgenic plants expressing the reporter of the uidA gene with the cis-elements.We extented the analysis of these transgenic plants for the pattern of the expession using in situ techniques.For the analysis of trans-factors, we used yeast expression systems. The ERECTA-5' region for regulated expression was fused to the E.coli lacZ gene and the reporter was introduced into yeast cells. Second plasmids carrying plant cDNAs with yeast promoter were introduced into the yeast cells with the ERECTA-lacZ reporter. After screening 3,000,000 colonies, we found 11 cDNAs for the expression of the reporter. One clone was isolated and has been shown to have same expression-chacteristics and DNA homology to DNA-transcription factors.Secondly, we extended the study of acaulis5 mutations. Our strategy is the map-based gene walking for the isolation of the ACAULIS5 gene. As an initial stetp, physical mapping was further extended in the chromosome 5 upper (south) -arm. We idetified the contigs using DNA clones included in the P1 phage-plasmids. One P1 clone is the possible candidate for the hit.