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Genetic Study of the Morphogenesis of Arabidopsis inflorescences.

拟南芥花序形态发生的遗传研究。

基本信息

批准号：
05454004
负责人：
KOMEDA Yoshibumi
金额：
$ 3.97万
依托单位：
Hokkaido University (1994)The University of Tokyo (1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

a) Arabidopsis thaliana morphological mutants were extensively isolated which have defects in the process of the inflorescence development. The relevant defects were ;i) Defective elongation of inflorescences.ii) A berrant morphology in the tip of inflorescences.In summary, more than 30 mutant lines were identified.b) The mutants were genetically crossed with known morphological mutants and with each other. They were divided as follows ;i) Mutations which are defective in the elongation of inflorescences.acaulis (acl) 1, ac12, ac13, ac14, ac15ii) Mutations whose tips of inflorescences are not racemose but corymbose-form.erecta (er), corymbosa (crm) 1, crm2These loci but er were isolated in this study. The mutation in erecta locus used to be the only one allele, but we newly isolated more than 5 alleles.c) We have located these loci on the standard Arabidpsis genetic map.d) We examined the morphology of the meristematic tissues of these mutants by scanning electron microscopy. Apparent abnormatity was not detected in these mutants.e) The effects of exogenously applied phytohormones were examined. No apparent/dorastic influence was detected by this treatment. In order to perturbe the internal hormone, additional mutations which involved in phytohormone metabolism were introduced. The examinatiion of this double mutants are under way.f) The fine molecular/ohysical map of the ac12 locus was constructed.

A)大量分离到在花序发育过程中存在缺陷的拟南芥形态突变体。相关的缺陷是：1)花序伸长有缺陷；2)花序顶端形态异常。总之，共鉴定出30多个突变系。b)这些突变体与已知的形态突变体以及彼此之间进行了遗传杂交。1)花序伸长缺陷突变。acaulis(Acl)1，ac12，ac13，ac14，ac15。ii)花序顶端不是总状花序而是伞房花序状的突变。erecta(Er)，corymbosa(CRM)1，crm2。直立位的突变曾经是唯一的等位基因，但我们新分离到了5个以上的等位基因。c)我们在标准的拟南芥遗传图谱上定位了这些位点。d)我们用扫描电子显微镜观察了这些突变体的分生组织的形态。在这些突变体中没有检测到明显的异常。e)检测了外源植物激素的影响。这种处理没有检测到明显的/背部的影响。为了扰乱植物体内的激素，引入了与植物激素代谢有关的额外突变。这一双突变体的检测正在进行中。f)构建了AC12基因座的精细分子/物理图谱。